Abstract
Despite enormous efforts in treating myocardial infarction (MI) and subsequent heart failure, the recent statistics from the American Heart Association evidently show that there still remains room for improvements. To develop and translate new therapeutics toward clinics, large animal models that allow us to test new therapies in human-like conditions are of extraordinary importance. In this chapter, we describe detailed protocols for the creation of a closed-chest MI model in pigs. The advantages of this model include high survival rate (>90% after ischemia–reperfusion), adjustable MI size depending on coronary occlusion site, reproducible cardiac dysfunction, and relatively low invasive method. The temporary coronary occlusion method for ischemia–reperfusion injury as well as the permanent occlusion method, using clot injection or embolic coil implantation, are described. Furthermore, we describe the key steps needed for understanding, performing, and analyzing cardiac angiography and echocardiography in pigs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jessup M, Brozena S (2003) Heart failure. N Engl J Med 348(20):2007–2018. https://doi.org/10.1056/NEJMra021498
Benjamin EJ, Blaha MJ, Chiuve SE, Cushman M, Das SR, Deo R, de Ferranti SD, Floyd J, Fornage M, Gillespie C, Isasi CR, Jimenez MC, Jordan LC, Judd SE, Lackland D, Lichtman JH, Lisabeth L, Liu S, Longenecker CT, Mackey RH, Matsushita K, Mozaffarian D, Mussolino ME, Nasir K, Neumar RW, Palaniappan L, Pandey DK, Thiagarajan RR, Reeves MJ, Ritchey M, Rodriguez CJ, Roth GA, Rosamond WD, Sasson C, Towfighi A, Tsao CW, Turner MB, Virani SS, Voeks JH, Willey JZ, Wilkins JT, Wu JH, Alger HM, Wong SS, Muntner P, American Heart Association Statistics Committee and Stroke Statistics Subcommittee (2017) Heart disease and stroke statistics-2017 update: a report from the American Heart Association. Circulation 135(10):e146–e603. https://doi.org/10.1161/CIR.0000000000000485
Houser SR, Margulies KB, Murphy AM, Spinale FG, Francis GS, Prabhu SD, Rockman HA, Kass DA, Molkentin JD, Sussman MA, Koch WJ, American Heart Association Council on Basic Cardiovascular Sciences, Council on Clinical Cardiology, Council on Functional Genomics and Translational Biology (2012) Animal models of heart failure: a scientific statement from the American Heart Association. Circ Res 111(1):131–150. https://doi.org/10.1161/RES.0b013e3182582523
Lecour S, Botker HE, Condorelli G, Davidson SM, Garcia-Dorado D, Engel FB, Ferdinandy P, Heusch G, Madonna R, Ovize M, Ruiz-Meana M, Schulz R, Sluijter JP, Van Laake LW, Yellon DM, Hausenloy DJ (2014) ESC working group cellular biology of the heart: position paper: improving the preclinical assessment of novel cardioprotective therapies. Cardiovasc Res 104(3):399–411. https://doi.org/10.1093/cvr/cvu225
Galvez-Monton C, Prat-Vidal C, Diaz-Guemes I, Crisostomo V, Soler-Botija C, Roura S, Llucia-Valldeperas A, Perea-Gil I, Sanchez-Margallo FM, Bayes-Genis A (2014) Comparison of two preclinical myocardial infarct models: coronary coil deployment versus surgical ligation. J Transl Med 12:137. https://doi.org/10.1186/1479-5876-12-137
McCall FC, Telukuntla KS, Karantalis V, Suncion VY, Heldman AW, Mushtaq M, Williams AR, Hare JM (2012) Myocardial infarction and intramyocardial injection models in swine. Nat Protoc 7(8):1479–1496. https://doi.org/10.1038/nprot.2012.075
Bangalore S, Bhatt DL (2011) Femoral arterial access and closure. Circulation 124(5):e147–e156. https://doi.org/10.1161/CIRCULATIONAHA.111.032235
Acknowledgments
This work is supported by NIH R01 HL139963 (K.I.), HL117505, HL 119046, HL129814, 128072, HL131404, HL135093, a P50 HL112324 (R.J.H.), AHA-SDG 17SDG33410873 (K.I.), and two Transatlantic Fondation Leducq grants. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health. O.B. was supported by the Deutsche Herzstiftung.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bikou, O., Watanabe, S., Hajjar, R.J., Ishikawa, K. (2018). A Pig Model of Myocardial Infarction: Catheter-Based Approaches. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_22
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8597-5_22
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8596-8
Online ISBN: 978-1-4939-8597-5
eBook Packages: Springer Protocols