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Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice

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The LINC Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1840))

Abstract

The linker of nucleoskeleton and cytoskeleton (LINC) complex mediates intracellular cross talk between the nucleus and the cytoplasm. In striated muscle, the LINC complex provides structural support to the myocyte nucleus and plays an essential role in regulating gene expression and mechanotransduction. A wide range of cardiac and skeletal myopathies have been linked to mutations in LINC complex proteins. Studies utilizing tissue-specific knockout and mutant mouse models have revealed important insights into the roles of the LINC complex in striated muscle. In this chapter, we describe several feasible approaches for generating striated muscle-specific gene knockout and mutant mouse models to study LINC complex protein function in cardiac and skeletal muscle. The experimental procedures used for phenotyping and analysis of LINC complex knockout mice are also described.

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Author notes

  1. Matthew J. Stroud

    Present address: Cardiovascular Division, King’s College London, British Heart Foundation Centre of Excellence, London, UK

  2. Matthew J. Stroud, Xi Fang and Jennifer Veevers contributed equally to this chapter.

Authors and Affiliations

  1. Department of Medicine, University of California, San Diego, La Jolla, CA, USA

    Matthew J. Stroud, Xi Fang, Jennifer Veevers & Ju Chen

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  1. Matthew J. Stroud
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  2. Xi Fang
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  3. Jennifer Veevers
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  4. Ju Chen
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Corresponding author

Correspondence to Ju Chen .

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Editors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University, New York, New York, USA

    Gregg G. Gundersen

  2. Department of Medicine and Department of Pathology and Cell Biology, Columbia University, New York, New York, USA

    Howard J. Worman

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Stroud, M.J., Fang, X., Veevers, J., Chen, J. (2018). Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice. In: Gundersen, G., Worman, H. (eds) The LINC Complex. Methods in Molecular Biology, vol 1840. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8691-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8691-0_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8690-3

  • Online ISBN: 978-1-4939-8691-0

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