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Illuminating Phytophthora Biology with Fluorescent Protein Tags

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Plant Pathogenic Fungi and Oomycetes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1848))

Abstract

Phytophthora species cause diseases that threaten agricultural, ornamental, and forest plants worldwide. Explorations of the biology of these pathogens have been aided by the availability of genome sequences, but much work remains to decipher the roles of their proteins. Insight into protein function can be obtained by visualizing them within cells, which has been facilitated by recent improvements in fluorescent protein and microscope technologies. Here, we describe strategies to permit investigators to generate strains of Phytophthora that express fluorescently tagged proteins and study their localization during growth in artificial media and during plant infection.

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Acknowledgments

This work has been supported by grants to H.S.J. from the National Institute of Food and Agriculture of the US Department of Agriculture, and the National Science Foundation.

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Authors and Affiliations

  1. Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA

    Audrey M. V. Ah-Fong, Meenakshi Kagda & Howard S. Judelson

Authors
  1. Audrey M. V. Ah-Fong
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  2. Meenakshi Kagda
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  3. Howard S. Judelson
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Corresponding author

Correspondence to Howard S. Judelson .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, CA, USA

    Wenbo Ma

  2. Oregon State University, Corvallis, OR, USA

    Thomas Wolpert

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Ah-Fong, A.M.V., Kagda, M., Judelson, H.S. (2018). Illuminating Phytophthora Biology with Fluorescent Protein Tags. In: Ma, W., Wolpert, T. (eds) Plant Pathogenic Fungi and Oomycetes. Methods in Molecular Biology, vol 1848. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8724-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8724-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8723-8

  • Online ISBN: 978-1-4939-8724-5

  • eBook Packages: Springer Protocols

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