Abstract
Cancer tissue engineering is an emerging multidisciplinary field aimed at growing cancerous cells onto porous biomaterial scaffolds and proper stimuli to ultimately reproduce 3D tumor tissue-like constructs in vitro. Unlike conventional 2D cell cultures and spheroids, these tissue models can reproduce cancer lesions very similar to those present in native tumor, and can be viable for some weeks, making it possible to study cancer biology phenomena and new therapies in a more reliable fashion than with conventional in vitro platforms. This chapter shows the preparation of a 3D model of pancreatic ductal adenocarcinoma (PDAC), including fabrication of a suitable scaffold, culture of PDAC cells on the scaffold, viability test, and histologic assessment.
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References
Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920–926
Fischbach C, Chen R, Matsumoto T, Schmelzle T, Brugge JS, Polverini PJ, Mooney DJ (2007) Engineering tumors with 3D scaffolds. Nat Methods 4:855–860
Ghajar CM, Bissell MJ (2010) Tumor engineering: the other face of tissue engineering. Tissue Eng Part A 16:2153–2156
Longati P, Jia X, Eimer J, Wagman A, Witt MR, Rehnmark S, Verbeke C, Toftgård R, Lohr M, Heuchel RL (2013) 3D pancreatic carcinoma spheroids induce a matrix-rich, chemoresistant phenotype offering a better model for drug testing. BMC Cancer 13:95
Aberle MR, Burkhart RA, Tiriac H, Olde Damink SWM, Dejong CHC, Tuveson DA, van Dam RM (2018) Patient-derived organoid models help define personalized management of gastrointestinal cancer. Br J Surg 105:e48–e60
Moscato S, Ronca F, Campani D, Danti S (2015) Poly(vinyl alcohol)/gelatin hydrogels cultured with HepG2 cells as a 3D model of hepatocellular carcinoma: a morphological study. J Funct Biomater 6:16–32
Ricci C, Moroni L, Danti S (2013) Cancer tissue engineering—new perspectives in understanding the biology of solid tumours—a critical review. OA Tissue Eng 1:4
Lamhamedi-Cherradi SE, Santoro M, Ramammoorthy V, Menegaz BA, Bartholomeusz G, Iles LR, Amin HM, Livingston JA, Mikos AG, Ludwig JA (2014) 3D tissue-engineered model of Ewing's sarcoma. Adv Drug Deliv Rev 79-80:155–171
Feig C, Gopinathan A, Neesse A, Chan DS, Cook N, Tuveson DA (2012) Pancreas cancer microenvironment. Clin Cancer Res 18:4266–4276
Funel N, Danti S, Salem AF, Pollina LE, Del Chiaro M, Pietrabissa A, Bevilacqua G, Mosca F, Boggi U, Campani D (2008) 3D in vitro model of pancreatic ductal adenocarcinoma: new strategy to study pancreatic ductal carcinoma. J Pancreas 9:810–811
Ricci C, Mota C, Moscato S, D'Alessandro D, Ugel S, Sartoris S, Bronte V, Boggi U, Campani D, Funel N, Moroni L, Danti S (2014) Interfacing polymeric scaffolds with primary pancreatic ductal adenocarcinoma cells to develop 3D cancer models. Biomatter 4:e955386
Chiellini F, Puppi D, Piras AM, Morelli A, Bartoli C, Migone C (2016) Modelling of pancreatic ductal adenocarcinoma in vitro with three-dimensional microstructured hydrogels. RSC Adv 59:54226–54235
Totti S, Vernardis SI, Meira L, Pérez-Mancera PA, Costello E, Greenhalf W, Palmer D, Neoptolemos J, Mantalaris A, Velliou EG (2017) Designing a bio-inspired biomimetic in vitro system for the optimization of ex vivo studies of pancreatic cancer. Drug Discov Today 22:690–701
Matta-Domjan B, King A, Totti S, Matta C, Dover G, Martinez P, Zakhidov A, La Ragione R, Macedo H, Jurewicz I, Dalton A, Velliou EG (2017) Biophysical interactions between pancreatic cancer cells and pristine carbon nanotube substrates: potential application for pancreatic cancer tissue engineering. J Biomed Mater Res B Appl Biomater. https://doi.org/10.1002/jbm.b.34012
Chew SA, Danti S (2017) Biomaterial-based implantable devices for cancer therapy. Adv Healthc Mater 6(2). doi: https://doi.org/10.1002/adhm.201600766
De la Ossa JG, Trombi L, D'Alessandro D, Coltelli MB, Serino LP, Pini R, Lazzeri A, Petrini M, Danti S (2017) Pore size distribution and blend composition affect in vitro prevascularized bone matrix formation on poly(vinyl alcohol)/gelatin sponges. Macromol Mater Eng 302:1700300
Barachini S, Danti S, Pacini S, D'Alessandro D, Carnicelli V, Trombi L, Moscato S, Mannari C, Cei S, Petrini M (2014) Plasticity of human dental pulp stromal cells with bioengineering platforms: a versatile tool for regenerative medicine. Micron 67:155–168
Acknowledgments
The authors acknowledge Dr. Delfo D’Alessandro (University of Pisa) for his technical contribution to histologic analysis.
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Ricci, C., Danti, S. (2019). 3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_8
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DOI: https://doi.org/10.1007/978-1-4939-8879-2_8
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