Abstract
Fermented foods produced using edible microorganisms have been consumed worldwide since ancient times. Microorganisms produce some healthy functional substances. Recently, many people have been attracted to the notion that d-body amino acids have healthy functional effects. d-body amino acids convert l-body amino acid proteolysis from a substrate such as foods during fermentation. This chapter presents a description of methods used for d-alanine assays in the solution as medium and AARase assays in lactic acid bacteria (LAB) cells for isolation of high AARase-containing strains using d-amino acid oxidase and lactic acid dehydrogenase via a NADH oxidoreduction system.
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References
Mutaguchi Y, Ohmori T, Ohshima T (2015) Lactic fermentation and d-amino acid production. Kagaku to Seibutsu 53:18–26
Gogami Y, Okada K, Moriyama M et al (2012) Quantitative analysis of d-amino acids in sake brewing processes of Kimoto, Kimoto adding starter lactic acid bacteria, and Sokujomoto. Trace Nutr Res 29:1–6 in Japanese
Ashida Y, Tojo Y, Shimada S et al (2011) Presence of free d-amino acids in the skin and their physiological functions. Bio Industry 28:40–44
Inoue Y, Okabe Y, Suzuki R et al (2014) Effect of d-amino acids as taste modifiers in fermented foods. Trace Nutr Res 31:59–65 in Japanese
Mutaguchi Y, Ohmori T, Akamatsu T et al (2013) Identification, purification, and characterization of a novel amino acid racemase, isoleucine 2-epimerase, from lactobacillus species. J Bacteriol 195(22):5207–5215
Kato S, Oikawa T (2015) Genome analysis of lactic acid bacteria: current status and perspective of d-amino acid studies. Trace Nutr Res 32:78–82
Uo T, Yoshimura T, Tanaka N et al (2001) Functional characterization of alanine racemase from Schizosaccaromyces pombe: a eucaryotic counterpart to bacterial alanine racemase. J Bacteriol 7:2226–2233
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Matsumoto, N., Kanauchi, M. (2019). Assaying d-Body Amino Acids as d-Alanine and Amino Acid Racemase (AARase) Activity Using NADH Oxidoreduction Enzymic System. In: Kanauchi, M. (eds) Lactic Acid Bacteria. Methods in Molecular Biology, vol 1887. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8907-2_3
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DOI: https://doi.org/10.1007/978-1-4939-8907-2_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8906-5
Online ISBN: 978-1-4939-8907-2
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