Skip to main content
SpringerLink
Log in

Bioengineering Tooth Bud Constructs Using GelMA Hydrogel

  • Protocol
  • First Online:
Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

Bioengineered dental tissues and whole teeth that exhibit features and properties of natural teeth can functionally surpass currently used artificial dental implants. However, no biologically based alternatives currently exist for clinical applications in dentistry. Here, we describe a newly established bioengineered tooth bud model for eventual applications in clinical dentistry. We also describe methods to fabricate and analyze bioengineered tooth tissues, including cell isolation, in vivo implantation, and post-harvest analyses.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Greenstein G, Cavallaro J, Romanos G, Tarnow D (2008) Clinical recommendations for avoiding and managing surgical complications associated with implant dentistry: a review. J Periodontol 79:1317–1329

    Article  Google Scholar 

  2. Jung RE, Pjetursson BE, Glauser R, Zembic A, Zwahlen M, Lang NP (2008) A systematic review of the 5-year survival and complication rates of implant-supported single crowns. Clin Oral Implants Res 19:119–130

    Article  Google Scholar 

  3. Chrcanovic BR, Albrektsson T, Wennerberg A (2014) Reasons for failures of oral implants. J Oral Rehabil 41:443–476

    Article  CAS  Google Scholar 

  4. Chrcanovic BR, Kisch J, Albrektsson T, Wennerberg A (2016) Factors influencing early dental implant failures. J Dent Res 95:995–1002

    Article  CAS  Google Scholar 

  5. Yen AH, Yelick PC (2011) Dental tissue regeneration – a mini-review. Gerontology 57:85–94

    Article  Google Scholar 

  6. Smith EE, Yelick PC (2016) Progress in bioengineered whole tooth research: from bench to dental patient chair. Curr Oral Health Rep 3(4):302–308

    Article  Google Scholar 

  7. Lai W-F, Lee J-M, Jung H-S (2014) Molecular and engineering approaches to regenerate and repair teeth in mammals. Cell Mol Life Sci 71:1691–1701

    Article  CAS  Google Scholar 

  8. Monteiro N, Smith EE, Angstadt S, Zhang W, Khademhosseini A, Yelick PC (2016) Dental cell sheet biomimetic tooth bud model. Biomaterials 106:167–179

    Article  CAS  Google Scholar 

  9. Smith EE, Zhang W, Schiele NR, Khademhosseini A, Kuo CK, Yelick PC (2017) Developing a biomimetic tooth bud model. J Tissue Eng Regen Med 11(12):3326–3336

    Article  CAS  Google Scholar 

  10. Monteiro N, Yelick PC (2016) Advances and perspectives in tooth tissue engineering. J Tissue Eng Regen Med 11(9):2443–2461

    Article  Google Scholar 

  11. Yue K, Santiago GT-d, Alvarez MM, Tamayol A, Annabi N, Khademhosseini A (2015) Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73:254–271

    Article  CAS  Google Scholar 

  12. Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A (2010) Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials 31:5536–5544

    Article  CAS  Google Scholar 

Download references

Acknowledgments

All members of the Yelick Tissue Engineering Lab have contributed to optimize and validate these techniques. This work was supported by NIH/NIDCR R01 DE16132 (PCY) and NIH/NIDCR F31 DE026361 (EES).

Author information

Authors and Affiliations

  1. Department of Cell, Molecular, and Developmental Biology, Sackler School of Graduate Biomedical Sciences, Tufts University School Medicine, Boston, MA, USA

    Elizabeth E. Smith

  2. Department of Orthodontics, Tufts University School of Dental Medicine, Boston, MA, USA

    Pamela C. Yelick

Authors
  1. Elizabeth E. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pamela C. Yelick
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pamela C. Yelick .

Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Smith, E.E., Yelick, P.C. (2019). Bioengineering Tooth Bud Constructs Using GelMA Hydrogel. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation