Abstract
An important issue in tissue biomechanics is to decipher the relationship between the mechanical behavior at macroscopic scale and the organization of the collagen fiber network at microscopic scale. Here, we present a protocol to combine traction assays with multiphoton microscopy in ex vivo murine skin. This multiscale approach provides simultaneously the stress/stretch response of a skin biopsy and the collagen reorganization in the dermis by use of second harmonic generation (SHG) signals and appropriate image processing.
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Acknowledgments
This work was supported by grants from Ecole Polytechnique (interdisciplinary project) and from Agence Nationale de la Recherche (contracts ANR-10-INBS-04 France BioImaging, ANR-11-EQPX-0029 Morphoscope2, and ANR-13-BS09-0004-02 Metis). The authors thank Vincent de Greef for his help in the technical implantation of the mechanical setup.
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Bancelin, S. et al. (2019). Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics. In: Sagi, I., Afratis, N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9095-5_11
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DOI: https://doi.org/10.1007/978-1-4939-9095-5_11
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