Abstract
I2CITC allows the analysis of isothermal titration calorimetry (ITC) data for complex coupled equilibria. Here we describe how, using I2CITC, ITC data for systems involving a self-aggregating ligand and a host offering one or two binding sites can be analyzed, how interaction models can be tested, and how confidence intervals for the optimized parameters can be determined.
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Users of ICITC and I2CITC, past and present, are thanked for their feedback and questions which have contributed to the improvement of the software over the years.
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Saeed, I.Q., Buurma, N.J. (2019). Analysis of Isothermal Titration Calorimetry Data for Complex Interactions Using I2CITC. In: Ennifar, E. (eds) Microcalorimetry of Biological Molecules. Methods in Molecular Biology, vol 1964. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9179-2_13
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DOI: https://doi.org/10.1007/978-1-4939-9179-2_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-9179-2
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