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Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

Abstract

Analysis of meristem shape and gene expression pattern has been conducted in many species over the past decades. Recent live imaging techniques have allowed for an unprecedented accumulation of data on the biology of meristematic cells, as well as a better understanding of the molecular and biophysical mechanisms behind shape changes in this tissue. Here we describe in detail how to prepare shoot apices of both Arabidopsis and tomato, in order to image them over time using a confocal microscope equipped with a long distance water-dipping lens.

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Acknowledgments

We dedicate this chapter to Olivier Grandjean who initiated the live imaging of meristems in 2004, while in the group of Jan Traas [8]. This work was supported by a bilateral grant from INRA, France and Ministry of Science and Higher Education, Poland and by a grant from Agence Nationale de la Recherche ANR-10-BLAN-1516 «Mechastem», and a research grant SONATA BIS (2016/22/E/NZ3/00342) from the National Science Centre, Poland (to A.B.).

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Authors and Affiliations

  1. Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, UCB Lyon 1, ENS de Lyon, INRA, CNRS, Lyon, France

    Olivier Hamant & Pradeep Das

  2. Department of Biophysics and Morphogenesis of Plants, University of Silesia in Katowice, Katowice, Poland

    Agata Burian

Authors
  1. Olivier Hamant
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  2. Pradeep Das
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  3. Agata Burian
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Corresponding author

Correspondence to Olivier Hamant .

Editor information

Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Hamant, O., Das, P., Burian, A. (2019). Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

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