Abstract
Bioluminescence imaging (BLI) has become a major strategy for real-time analysis of dynamic biological processes. In particular, bioluminescent reporter microorganisms have been designed to advance our understanding of infectious diseases. Non-invasive monitoring of light-emitting pathogenic bacteria has revealed novel features of pathogenesis and enabled quantitative and qualitative analysis of antibacterial therapies. Transcriptional gene fusions using the bacterial luciferase operon luxCDABE as a reporter have been successfully used to monitor gene expression in vitro and in vivo, leading to valuable applications and major findings. In this chapter, we describe the construction of Yersinia pestis strains bearing a chromosomal copy of the luxCDABE operon under the control of promoters regulated by temperature and their application to quantify gene expression in real-time in bacteria growing in vitro and in a murine bubonic plague model.
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Acknowledgments
We thank Marie-Anne Nicola for her advice in the use of the bioluminescence system at the Photonic BioImaging facility of the Institut Pasteur.
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Derbise, A., Dussurget, O., Carniel, E., Pizarro-Cerdá, J. (2019). Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging. In: Vadyvaloo, V., Lawrenz, M. (eds) Pathogenic Yersinia. Methods in Molecular Biology, vol 2010. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9541-7_7
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DOI: https://doi.org/10.1007/978-1-4939-9541-7_7
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