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Intravital Imaging of Vaccinia Virus-Infected Mice

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Vaccinia Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2023))

Abstract

Intravital multiphoton microscopy (MPM) allows the direct visualization of viral infections in real time as they occur in living animals. Here we describe the routes and considerations for murine infection with vaccinia virus (VACV) for imaging, and the preparation of the skin and inner lip (labial mucosa) of infected animals for MPM. Using different recombinant VACVs expressing fluorescent proteins in combination with transgenic fluorescent reporter mice, MPM imaging can be used to examine the movements, interactions, and functions of virus-infected cells or selected immune cell populations after infection.

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Acknowledgments

This work was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. Ethan Tyler (NIH Medical Arts) created illustrations.

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Authors and Affiliations

  1. Viral Immunity and Pathogenesis Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    John P. Shannon, Glennys V. Reynoso & Heather D. Hickman

  2. Biological Imaging Section, Research Technology Branch, NIAID, NIH, Bethesda, MD, USA

    Olena Kamenyeva

Authors
  1. John P. Shannon
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  2. Olena Kamenyeva
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  3. Glennys V. Reynoso
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  4. Heather D. Hickman
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Corresponding author

Correspondence to Heather D. Hickman .

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Editors and Affiliations

  1. MRC-Laboratory for Molecular Cell Biology, University College London, London, UK

    Jason Mercer

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Shannon, J.P., Kamenyeva, O., Reynoso, G.V., Hickman, H.D. (2019). Intravital Imaging of Vaccinia Virus-Infected Mice. In: Mercer, J. (eds) Vaccinia Virus. Methods in Molecular Biology, vol 2023. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9593-6_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9593-6_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9592-9

  • Online ISBN: 978-1-4939-9593-6

  • eBook Packages: Springer Protocols

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