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Cell-Based Adhesion Assays for Isolation of Snake Venom’s Integrin Antagonists

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Snake and Spider Toxins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2068))

Abstract

Snake venoms could lead to the development of new drugs to treat a range of life-threatening conditions like cardiovascular diseases. Most snake venoms contain a large variety of lethal toxins as well as anti-adhesive proteins such as disintegrins, which have evolved from the harmless compounds ADAMs (proteins with a disintegrin and a metalloprotease domain) and C-type lectin proteins which disturb connective tissue and cell-matrix interaction. These anti-adhesive proteins target and block integrin receptors and disrupt normal biological processes in snakes’ prey such as connective tissue physiology and blood clotting. This chapter provides the experimental details of a practical, cell-based adhesion protocol to help identify and isolate disintegrins and C-type lectin proteins from snake venoms, important tools in integrin research and lead compounds for drug discovery.

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Acknowledgments

Philip Lazarovici holds the Jacob Gitlin Chair in Physiology and is affiliated with, and partially supported by, the Grass Center for Drug Design and Synthesis of Novel Therapeutics, David R. Bloom Center of Pharmacy, and the Adolph and Klara Brettler Medical Research Center at the Hebrew University of Jerusalem, Israel. Peter I Lelkes is the Laura H. Carnell Professor of Bioengineering.Cezary Marcinkiewicz and Peter I Lelkes acknowledge support through a grant from Temple University's Moulder Center for Drug Discovery.

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Authors and Affiliations

  1. Faculty of Medicine, School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel

    Philip Lazarovici

  2. Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA, USA

    Cezary Marcinkiewicz & Peter I. Lelkes

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  1. Philip Lazarovici
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  2. Cezary Marcinkiewicz
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  3. Peter I. Lelkes
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Correspondence to Philip Lazarovici .

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Editors and Affiliations

  1. Faculty of Medicine, School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel

    Avi Priel

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Lazarovici, P., Marcinkiewicz, C., Lelkes, P.I. (2020). Cell-Based Adhesion Assays for Isolation of Snake Venom’s Integrin Antagonists. In: Priel, A. (eds) Snake and Spider Toxins. Methods in Molecular Biology, vol 2068. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9845-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9845-6_11

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  • Publisher Name: Humana, New York, NY

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