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Expression Profiling of microRNAs in Cancer Cells: Technical Considerations

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Genomics Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 439))

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Abstract

MicroRNAs (miRNAs) represent a class of small, noncoding RNAs. These small RNAs are involved in diverse biological processes, and it has been predicted that about one third of human messenger RNAs (mRNAs) appear to be miRNA targets, underlying the major influence of miRNAs on almost all cellular pathways. Deviation from the normal pattern of miRNA expression has been implicated in several diseases. Among human diseases, it has been shown that changes in miRNA expression correlate with various human cancers. Thus, miRNA profiling could contribute to more precise tumor classification and better prediction of the therapeutic outcome. This chapter summarizes these recent findings and highlights the technical advances in miRNA probe preparation, miRNA expression profiling and target identification.

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Acknowledgments

We thank Dr. Anne Dybwad for her critical reading of the manuscript and Dr. Øystein Røsok for technical help. This work is supported in part by Helse Sør. The miRNA arrays were provided by the Norwegian Microarray Consortium at the Norwegian Radium Hospital.

Author information

Authors and Affiliations

  1. Department of Immunology, Institute for Cancer Research, Rikshopitalet — Radiumhospitalet Medical Center, Montebello, Oslo, Norway

    Mouldy Sioud

  2. Department of Tumor Biology, Institute for Cancer Research, Rikshopitalet—Radiumhospitalet Medical Center, Montebello, Oslo, Norway

    Lina Cekaite

Authors
  1. Mouldy Sioud
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  2. Lina Cekaite
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Editor information

Editors and Affiliations

  1. Animal Health Trust, Lanwades Park, Newmarket, Suffolk, UK

    Mike Starkey

  2. Oxford Gene Technology Ltd., Oxford, UK

    Ramnath Elaswarapu

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Sioud, M., Cekaite, L. (2008). Expression Profiling of microRNAs in Cancer Cells: Technical Considerations. In: Starkey, M., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 439. Humana Press. https://doi.org/10.1007/978-1-59745-188-8_12

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  • DOI: https://doi.org/10.1007/978-1-59745-188-8_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-871-3

  • Online ISBN: 978-1-59745-188-8

  • eBook Packages: Springer Protocols

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