Abstract
Protein fragment complementation has emerged as a powerful tool for measuring protein-protein interactions in the context of live cells. The adaptation of this strategy for use with firefly luciferase now allows for the non-invasive, quantitative, real-time readout of protein interactions in lysates, live cells, and whole animals. Bioluminescence provides a robust imaging modality due to its extremely low background signal and large dynamic range. The split luciferase fusion constructs described here are inducible by addition of ligands, small molecules or drugs, in this example, rapamycin, and have been shown to work in vivo.
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Acknowledgments
Special thanks to colleagues at the Molecular Imaging Center for valuable discussions. This educational project was supported by NIH grant P50 CA94056.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Villalobos, V., Naik, S., Piwnica-Worms, D. (2008). Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation. In: Starkey, M., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 439. Humana Press. https://doi.org/10.1007/978-1-59745-188-8_23
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DOI: https://doi.org/10.1007/978-1-59745-188-8_23
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