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Validation of RNAi by Real Time PCR

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RNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 703))

Abstract

Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.

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Author information

Authors and Affiliations

  1. The Bartholin Institute, Copenhagen University Hospital, Copenhagen, Denmark

    Knud Josefsen

  2. Cellular and Metabolic Research Section, Biomedical Institute, University of Copenhagen, Copenhagen, Denmark

    Ying C. Lee

Authors
  1. Knud Josefsen
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  2. Ying C. Lee
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Corresponding author

Correspondence to Knud Josefsen .

Editor information

Editors and Affiliations

  1. The Panum Institute, Dept. of Cellular & Molecular Medicine, University of Copenhagen, Blegdamsvej 3, Copenhagen, 2200N, Denmark

    Henrik Nielsen

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© 2011 Springer Science+Business Media, LLC

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Josefsen, K., Lee, Y.C. (2011). Validation of RNAi by Real Time PCR. In: Nielsen, H. (eds) RNA. Methods in Molecular Biology, vol 703. Humana Press. https://doi.org/10.1007/978-1-59745-248-9_14

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  • DOI: https://doi.org/10.1007/978-1-59745-248-9_14

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-913-0

  • Online ISBN: 978-1-59745-248-9

  • eBook Packages: Springer Protocols

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