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Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices

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Extracellular Matrix Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 522))

Summary

It is increasingly recognized that interactions between cancer cells and their surrounding stroma are critical for promoting the growth and invasiveness of tumors. For example, cancer cells alter the topography and molecular composition of stromal extracellular matrix by increasing paracrine regulation of fi-bro-blas-tic stromal cells during early tumor development. In turn, these physical and biochemical alterations of the stroma, profoundly affect the properties of the cancer cells. However, little is known about the cross-talk between stroma and cancer cells, and it is mainly due to the lack of a suitable in vitro system to mimic the stroma in vivo. We present an in vivo-like 3D stromal system derived from fibroblasts harvested from tissue samples representing various stages of stroma progression during tumorigenesis. The chapter describes how to isolate and characterize fibroblasts from a plethora of tissue samples. It describes how to produce and characterize fibroblast-derived 3D matrices. Finally, it describes how to test matrix permissiveness by analyzing the morphology of cancer cells cultured within various 3D matrices.

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Acknowledgments

We thank M. Valianou for critical comments and K. Buchheit for assertive proof reading. This work was supported by the American Association of Cancer Research (AACR) Pennsylvania Department of Health (the Department specifically disclaims responsibility for any analyses, interpretations, or conclusions), the National Institutes of Health/National Cancer Institute (grants CA006927, and RO1-CA113451), the Ovarian Cancer Research Foundation (OCRF), the W.W. Smith Charitable Trust and an appropriation from the Commonwealth of Pennsylvania.

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Authors and Affiliations

  1. Tumor Cell Biology-Basic Science, Fox Chase Cancer Center, Philadelphia, PA, 19111-2497, USA

    Edna Cukierman

Authors
  1. Remedios Castelló-Cros
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  2. Edna Cukierman
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Corresponding author

Correspondence to Edna Cukierman .

Editor information

Editors and Affiliations

  1. Gene Therapy Inst., Hadassah Univ. Hospital, Jerusalem, 91 120, Israel

    Sharona Even-Ram

  2. Lombardi Comprehensive Cancer Ctr., Georgetown University, Reservoir Rd. NW., 3970, Washington, 20057-1468, U.S.A.

    Vira Artym

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Castelló-Cros, R., Cukierman, E. (2009). Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices. In: Even-Ram, S., Artym, V. (eds) Extracellular Matrix Protocols. Methods in Molecular Biology, vol 522. Humana Press. https://doi.org/10.1007/978-1-59745-413-1_19

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  • DOI: https://doi.org/10.1007/978-1-59745-413-1_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-984-0

  • Online ISBN: 978-1-59745-413-1

  • eBook Packages: Springer Protocols

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