Abstract
Wave Bioreactors are relatively new to the field of protein production from insect cells infected with recombinant baculoviruses. Various sizes are available to support expression needs from small to large scale. They offer many advantages over stirred tank and airlift reactors, including simple operation, ease of setup and clean-up, and minimal utility requirements. The design consists of a platform rocker onto which presterilized and assembled bags are placed. Once inflated, the bags are filled with medium and cells. Filtered air flow maintains the volumetric shape of the bag and provides head space for gas exchange. A “wave” motion is created by the rocking and angle settings of the platform. Monitoring can be as simple or as detailed as the user requires. Excellent insect cell growth and production of proteins from many classes can be achieved with these units.
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References
Singh, V. (1999) Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology 30, 149–158.
Kost, T. A., Condreay, J. P., and Jarvis, D. J. (2005) Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat. Biotechnol. 23, 567–575.
http://www.wavebiotech.com/products/wave_bioreactor/literature.html and http://www.wavebiotech.ch/site_v2 sections give many references for the uses of Wave Bioreactors.
Ohashi, R., Singh, V., and Hamel, J-F. (2001) Perfusion cell culture in disposable bioreactors. Presented in Animal Cell Technology: from Target to Market, Proceeding from the 17th ESACT Meeting, Tylösand, Sweden, June 10–14th 2001, pp. 403–409. Edited by E. Lindner-Olsson, N. Chatzissavidou, E. Lüllau, Kluwer Academic Pub.
Ohashi, R. and Hamel, J-F. (2003) Perfusion culture in the wave reactor for the production of a monoclonal antibody. 8th Waterside Conference, San Francisco, CA.
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© 2007 Humana Press Inc., Totowa, NJ
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Kadwell, S.H., Hardwicke, P.I. (2007). Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors. In: Murhammer, D.W. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology™, vol 388. Humana Press. https://doi.org/10.1007/978-1-59745-457-5_12
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DOI: https://doi.org/10.1007/978-1-59745-457-5_12
Publisher Name: Humana Press
Print ISBN: 978-1-58829-537-8
Online ISBN: 978-1-59745-457-5
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