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Lepidopteran Insect Cell Line Isolation from Insect Tissue

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Baculovirus and Insect Cell Expression Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 388))

Abstract

This chapter describes procedures for initiating new cell lines from lepidopteran larval tissues. The internal morphology is described along with methods for treating excised tissues and the primary cultures. Advice on culture medium and the tissues that will provide the best chance for new cell lines is discussed.

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References

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© 2007 Humana Press Inc., Totowa, NJ

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Lynn, D.E. (2007). Lepidopteran Insect Cell Line Isolation from Insect Tissue. In: Murhammer, D.W. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology™, vol 388. Humana Press. https://doi.org/10.1007/978-1-59745-457-5_7

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  • DOI: https://doi.org/10.1007/978-1-59745-457-5_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-537-8

  • Online ISBN: 978-1-59745-457-5

  • eBook Packages: Springer Protocols

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