Summary
Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases, electrophoresis in the presence of positively charged detergents like cetyltrimethylammonium bromide may work considerably better. Methods for electrophoresis, staining, and blotting of such gels are presented.
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This work was supported in part by Kuwait University grant MPB029.
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Buxbaum, E. (2009). Cationic electrophoresis and eastern blotting . In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_14
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DOI: https://doi.org/10.1007/978-1-59745-542-8_14
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