Abstract
The immobilization procedure to a biosensor surface has a major influence on the measurement results. To characterize the immobilization onto various biolayers, the interaction of DNA anti-thrombin aptamer with the protein thrombin was used as a model system. The aptamer was immobilized to a two-dimensional alkanethiol SAM via carboxylamide bonds and to a three-dimensional dextran matrix via streptavidin–biotin interaction. The calculated K D values of about 260 and 267 nM, respectively, were comparable, while the amount of bound analyte varied by a factor of 2, depending on the accessibility of the immobilized aptamer. Differences in the specificity were shown by use of the similar protein elastase.
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Acknowledgments
I would like to thank Antje Baumgartner for technical assistance and Ulrich Schlecht for helpful discussions.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Gronewold, T.M.A. (2009). Aptamers and Biosensors. In: Mayer, G. (eds) Nucleic Acid and Peptide Aptamers. Methods in Molecular Biology™, vol 535. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-557-2_13
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DOI: https://doi.org/10.1007/978-1-59745-557-2_13
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