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Modeling Substance Abuse for Applications in Proteomics

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Neuroproteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 566))

Summary

The ability to model aspects of human addictive behaviors in laboratory animals provides an important avenue for gaining insight into the biochemical alterations associated with drug intake and the identification of targets for medication development to treat addictive disorders. The intravenous self-administration procedure provides the means to model the reinforcing effects of abused drugs and to correlate biochemical alterations with drug reinforcement. In this chapter, we provide a detailed methodology for rodent intravenous self-administration and the isolation and preparation of proteins from dissected brain regions for Western blot analysis and high-throughput proteomic analysis. Examples of cocaine-induced alterations in the abundances of ionotropic glutamate receptor subunits in reinforcement-related brain regions are provided.

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Acknowledgments

The preparation of this chapter was funded in part by DA012498, DA06634, and DA03628 (SEH).

Author information

Authors and Affiliations

  1. Departments of Physiology & Pharmacology and Psychiatry, Wake Forest University School of Medicine, Winston-Salem, NC, USA

    Scott E. Hemby & Nilesh Tannu

Authors
  1. Scott E. Hemby
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  2. Nilesh Tannu
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Corresponding author

Correspondence to Scott E. Hemby .

Editor information

Editors and Affiliations

  1. Dept. Anatomy & Neurobiology, Virginia Commonwealth University School of Medicine, Richmond, U.S.A.

    Andrew K. Ottens

  2. Center of Innovative Research, Banyan Biomarkers, Inc. Center of Innovative Research, Alachua, U.S.A.

    Kevin K.W. Wang

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Hemby, S.E., Tannu, N. (2009). Modeling Substance Abuse for Applications in Proteomics. In: Ottens, A., Wang, K. (eds) Neuroproteomics. Methods in Molecular Biology, vol 566. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-562-6_5

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  • DOI: https://doi.org/10.1007/978-1-59745-562-6_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-84-8

  • Online ISBN: 978-1-59745-562-6

  • eBook Packages: Springer Protocols

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