Summary
Phosphorylation is one of the most abundant post-translational modifications on protein and one that frequently has functional biological consequences. For this reason, screening protein samples for phosphorylations has become an important tool in biochemical research. Affinity purification by immunological or chemical reagents can be used to isolate phosphoproteins from other cellular materials.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Puente, L.G., Megeney, L.A. (2008). Isolation of Phosphoproteins. In: Posch, A. (eds) 2D PAGE: Sample Preparation and Fractionation. Methods in Molecular Biology™, vol 424. Humana Press. https://doi.org/10.1007/978-1-60327-064-9_28
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DOI: https://doi.org/10.1007/978-1-60327-064-9_28
Publisher Name: Humana Press
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