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References
Chalfie, M., Tu, Y., Euskirchen, G, Ward, W.W., and Prasher, D.C. Green fluorescent protein as a marker for gene expression. Science, 1994; 263: 802–805.
Utermark, J., and Karlovsky, P. Quantification of green fluorescent protein fluorescence using real-time PCR thermal cycler. Biotechniques, 2006; 41: 152–154.
Spergel, D.J., Kruth, U., Shimshek, D.R., Sprengel, R., and Seeburg, P.H. Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies. Prog. Neurobiol., 2001; 63: 673–686.
Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T., and Nishimune, Y. ‘‘Green mice’’ as a source of ubiquitous green cells. FEBS Lett., 1997; 407: 313–319.
Balthasar, N., Mery, P.F., Magoulas, C.B., Mathers, K.E., Martin, A., Mollard, P., and Robinson, I.C. Growth hormone-releasing hormone (GHRH) neurons in GHRH-enhanced green fluorescent protein transgenic mice: A ventral hypothalamic network. Endocrinology, 2003; 144: 2728–2740.
Sugino, K., Hempel, C.M., Miller, M.N.,Hattox, A.M., Shapiro, P., Wu, C., Huang, Z.J., and Nelson, S.B. Molecular taxonomy of major neuronal classes in the adult mouse forebrain. Nat. Neurosci., 2006; 9: 99–107.
Burke, N.V., Han, W., Li, D., Takimoto, K., Watkins, S.C.,and Levitan, E.S. Neuronal peptide release is limited by secretory granule mobility. Neuron, 1997; 19: 1095–1102.
Young, W.S., Iacangelo, A., Luo, X.Z., King, C., Duncan, K., and Ginns, E.I. Transgenic expression of green fluorescent protein in mouse oxytocin neurons. J. Neuroendocrinol., 1999; 11: 935–939.
Davies, J.S., Holter, J.L., Knight, D., Beaucourt, S.M., Murphy, D., Carter, D.A., and Wells, T. Manipulating sorting signals to generate co-expression of somatostatin and eGFP in the regulated secretory pathway from a monocistronic construct. J. Mol. Endocrinol., 2004; 33: 523–532.
Houdebine, L.M., and Attal, J. Internal ribosome entry sites (IRESs): Reality and use. Transgenic Res., 1999; 8: 157–177.
Krestel, H.E., Mayford, M., Seeburg, P.H., and Sprengel, R. A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse. Nucleic Acids Res., 2001; 29: E39.
Zhang, B.J., Kusano, K., Zerfas, P., Iacangelo, A.,Young, W.S., and Gainer, H. Targeting of green fluorescent protein to secretory granules in oxytocin magnocellular neurons and its secretion from neurohypophysial nerve terminals in transgenic mice. Endocrinology, 2002; 143: 1036–1046.
Transgenesis techniques. In Methods in Molecular Biology, Vol. 18, D. Murphy and D.A. Carter, eds. Humana Press, Totowa, NJ, 1993.
Slade, J.P., Man, P.-S., Wells, T., and Carter, D.A. Stimulus-specific induction of an egr-1 transgene in rat brain. Neuroreport, 2001; 13: 671–674.
Man, P.-S., and Carter, D.A. Oestrogenic regulation of an egr-1 transgene in rat anterior pituitary. J. Mol. Endocr., 2002; 30: 187–193.
Smith, M., Burke, Z., Humphries, A., Wells, T., Klein, D., Carter, D., and Baler, R. Tissue-specific transgenic knockdown of Fos-related antigen 2 (Fra-2) expression mediated by dominant negative Fra-2. Mol. Cell. Biol., 2001; 21: 3704–3713.
Shariatmadari, R., Sipila, P.P., Huhtaniemi, I.T., and Poutanen, M. Improved technique for detection of enhanced green fluorescent protein in transgenic mice. Biotechniques, 2001; 30: 1282–1285.
van den Pol, A.N., and Ghosh, P.K. Selective neuronal expression of green fluorescent protein with cytomegalovirus promoter reveals entire neuronal arbor in transgenic mice. J. Neurosci., 1998; 18: 10640–10651.
Magoulas, C., McGuinness, L., Balthasar, N., Carmignac, D.F., Sesay, A.K., Mathers, K.E., Christian, H., Candeil, L., Bonnefont, X., Mollard, P., and Robinson, I.C. A secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice. Endocrinology, 2000; 141: 4681–4689.
Carter, D, Cellular transcriptomics—The next phase of endocrine expression profiling. Trends Endocrinol. Metab., 2006; 17: 192–198.
Lobo, M.K., Karsten, S.L., Gray, M., Geschwind, D.H., and Yang, X.W. FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brains. Nat. Neurosci., 2006; 9: 443–452.
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Holter, J., Davies, J., Man, PS., Wells, T., Carter, D. (2008). Transgenic Delivery and Detection of GFP in Neuropeptide Neurons. In: Gozes, I. (eds) Neuropeptide Techniques. Neuromethods, vol 39. Humana Press. https://doi.org/10.1007/978-1-60327-099-1_5
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DOI: https://doi.org/10.1007/978-1-60327-099-1_5
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