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Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells

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Protocols for Adult Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 621))

Abstract

In our studies of muscle regeneration we have developed, modified, and optimized techniques to isolate and study the stem and precursor cells to muscle tissue. Our goals have been to obtain for study muscle fibers in bulk, or the fiber-associated cells, separately from the other cells found in muscle. Using these techniques, myofiber-associated cells may be isolated from neonatal through adult muscle, from resting or from regenerating muscle, thus allowing one to investigate the cellular populations participating during the time course of these events. The protocol is applicable to any age and condition of muscle and may be adapted for other tissues.

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Acknowledgements

We would like to thank members of the Conboy and Wagers laboratories for constructive feedback on these protocols, John Wang for assistance with Fig. 2, and Matthew O’Connor for tips on magnetic bead sorting. We thank the National Institutes of Health, National Institute on Aging (R01 AG027252), the Stem Cell Research Foundation/American Health Assistance Foundation, California Institute for Regenerative Medicine and Ellison’s Medical Foundation support to I.M.C.

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Authors and Affiliations

  1. Department of BioEngineering, University of California, Berkeley, CA, USA

    Michael J. Conboy & Irina M. Conboy

Authors
  1. Michael J. Conboy
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  2. Irina M. Conboy
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Corresponding author

Correspondence to Irina M. Conboy .

Editor information

Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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Conboy, M.J., Conboy, I.M. (2010). Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_10

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_10

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

  • eBook Packages: Springer Protocols

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