Abstract
Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thiols, or noncovalent immobilization using high-affinity interactions such as biotin/streptavidin or antibody/antigen. In this chapter we describe a robust method to covalently immobilize His6 fusion proteins on the sensor surface for SPR analysis.
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Kimple, A.J., Muller, R.E., Siderovski, D.P., Willard, F.S. (2010). A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance. In: Mol, N., Fischer, M. (eds) Surface Plasmon Resonance. Methods in Molecular Biology, vol 627. Humana Press. https://doi.org/10.1007/978-1-60761-670-2_5
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DOI: https://doi.org/10.1007/978-1-60761-670-2_5
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