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Structural Approaches of the Mitochondrial Carrier Family

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Membrane Protein Structure Determination

Part of the book series: Methods in Molecular Biology ((MIMB,volume 654))

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Abstract

The transport of solutes across the inner mitochondrial membrane is highly selective and necessitates membrane proteins mainly from the mitochondrial carrier family (MCF). These carriers are required for the transport of a variety of metabolites implicated in all the important processes occurring within the mitochondrial matrix. Due to its high abundance, the ADP/ATP carrier (AAC) is the member of the family that was studied most. It is the first mitochondrial carrier for which a high-resolution X-ray structure is known. The carrier was crystallized in the presence of a strong inhibitor, the carboxyatractyloside (CATR). The structure gives an insight not only into the overall fold of mitochondrial carriers in general but also into atomic details of the AAC in a conformation that is open toward the intermembrane space (IMS). Molecular dynamics simulations indicate the first events occurring to the carrier after the binding of ADP. A careful analysis of the primary sequences of all the carriers in light with the structure highlights properties of the protein that are related to the substrate.

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Author information

Authors and Affiliations

  1. Institut Pasteur, Unit if Structural Dynamics of Macromolecules, CNRS, URA 2185, Paris, France

    Hugues Nury

  2. Institut de Biologie Structurale, CEA-CNRS and Université Joseph Fourier, Grenoble, France

    Iulia Blesneac, Stephanie Ravaud & Eva Pebay-Peyroula

Authors
  1. Hugues Nury
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  2. Iulia Blesneac
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  3. Stephanie Ravaud
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  4. Eva Pebay-Peyroula
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Corresponding author

Correspondence to Eva Pebay-Peyroula .

Editor information

Editors and Affiliations

  1. , Université Paris Diderot - Paris 7, INSERM U773/CRB3, rue Henri Huchard 16, Paris, 75018, France

    Jean-Jacques Lacapère

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Nury, H., Blesneac, I., Ravaud, S., Pebay-Peyroula, E. (2010). Structural Approaches of the Mitochondrial Carrier Family. In: Lacapère, JJ. (eds) Membrane Protein Structure Determination. Methods in Molecular Biology, vol 654. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-762-4_6

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  • DOI: https://doi.org/10.1007/978-1-60761-762-4_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-761-7

  • Online ISBN: 978-1-60761-762-4

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