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NMR Spectroscopy to Study the Dynamics and Interactions of CFTR

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Cystic Fibrosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 741))

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-domain membrane chloride channel whose activity is regulated by ATP at two nucleotide-binding domains (NBD1 and NBD2) and by phosphorylation of the regulatory (R) region. The NBDs and the R region have functionally relevant motions that are critical for channel gating. Nuclear magnetic resonance (NMR) spectroscopy is a highly useful technique for obtaining information on the structure and interactions of CFTR and is extremely powerful for probing dynamics. NMR approaches for studying CFTR are reviewed, using our previous NBD1 and the R region results to provide examples. These NMR data are yielding insights into the dynamic properties and interactions that facilitate normal CFTR regulation as well as pathological effects of mutations, including the most common disease mutant, deletion of F508 in NBD1.

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Authors and Affiliations

  1. Department of Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, ON, Canada

    Voula Kanelis

  2. Molecular Structure and Function, Hospital for Sick Children, M5G1X8, Toronto, ON, Canada

    P. Andrew Chong & Julie D. Forman-Kay

  3. Department of Biochemistry, University of Toronto, Toronto, ON, Canada

    Julie D. Forman-Kay

Authors
  1. Voula Kanelis
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  2. P. Andrew Chong
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  3. Julie D. Forman-Kay
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Correspondence to Voula Kanelis .

Editor information

Editors and Affiliations

  1. Faculty of Sciences, Centre for Biodiversity & Functional, University of Lisboa, Campo Grande, Lisboa, 1749-016, Portugal

    Margarida D. Amaral

  2. , Department of Physiology, University of Regensburg, Universitätsstr. 31, Regensburg, 93053, Germany

    Karl Kunzelmann

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Kanelis, V., Chong, P.A., Forman-Kay, J.D. (2011). NMR Spectroscopy to Study the Dynamics and Interactions of CFTR. In: Amaral, M., Kunzelmann, K. (eds) Cystic Fibrosis. Methods in Molecular Biology, vol 741. Humana Press. https://doi.org/10.1007/978-1-61779-117-8_25

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  • DOI: https://doi.org/10.1007/978-1-61779-117-8_25

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-116-1

  • Online ISBN: 978-1-61779-117-8

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