Abstract
Homologous recombination is an important pathway for error-free repair of DNA lesions, such as single- and double-strand breaks, and for rescue of collapsed replication forks. Here, we describe protocols for live cell imaging of single-lesion recombination events in the yeast Saccharomyces cerevisiae using fluorescence microscopy.
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Acknowledgments
The LacI-R197K mutant protein was engineered by Christian Müller. This work was supported by The Danish Agency for Science, Technology and Innovation (ML), the Villum Kann Rasmussen Foundation (ML), GM67055 (RR), and the Lundbeck Foundation (NEB).
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Eckert-Boulet, N., Rothstein, R., Lisby, M. (2011). Cell Biology of Homologous Recombination in Yeast. In: Tsubouchi, H. (eds) DNA Recombination. Methods in Molecular Biology, vol 745. Humana Press. https://doi.org/10.1007/978-1-61779-129-1_30
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DOI: https://doi.org/10.1007/978-1-61779-129-1_30
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