Abstract
In recent years, several reporter genes have been designed for non-invasive magnetic resonance imaging (MRI). Here, we offer a brief summary of recent advances in MRI reporter gene technology, as well as elaborated protocols for cloning, expression, and imaging of reporter genes based on a chemical exchange saturation transfer (CEST) method. These protocols emphasize new developments in CEST-MRI data acquisition and processing.
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References
Serganova, I., Ponomarev, V., and Blasberg, R. (2007) Human reporter genes: potential use in clinical studies. Nucl Med Biol 34, 791–807.
Koretsky, A. P., Brosnan, M. J., Chen, L. H., Chen, J. D., and Van Dyke, T. (1990) NMR detection of creatine kinase expressed in liver of transgenic mice: determination of free ADP levels. Proc Natl Acad Sci USA 87, 3112–3116.
Walter, G., Barton, E. R., and Sweeney, H. L. (2000) Noninvasive measurement of gene expression in skeletal muscle. Proc Natl Acad Sci USA 97, 5151–5155.
Alfke, H., Stoppler, H., Nocken, F., Heverhagen, J. T., Kleb, B., Czubayko, F., and Klose, K. J. (2003) In vitro MR imaging of regulated gene expression. Radiology 228, 488–492.
Weissleder, R., Moore, A., Mahmood, U., Bhorade, R., Benveniste, H., Chiocca, E. A., and Basilion, J. P. (2000) In vivo magnetic resonance imaging of transgene expression. Nat Med 6, 351–355.
Cohen, B., Ziv, K., Plaks, V., Israely, T., Kalchenko, V., Harmelin, A., Benjamin, L. E., and Neeman, M. (2007) MRI detection of transcriptional regulation of gene expression in transgenic mice. Nat Med 13, 498–503.
Genove, G., Demarco, U., Xu, H., Goins, W. F., and Ahrens, E. T. (2005) A new transgene reporter for in vivo magnetic resonance imaging. Nat Med 4, 450–454.
Kodibagkar, V. D., Yu, J., Liu, L., Hetherington, H. P., and Mason, R. P. (2006) Imaging beta-galactosidase activity using (19)F chemical shift imaging of LacZ gene-reporter molecule 2-fluoro-4-nitrophenol-beta-d-galactopyranoside. Magn Reson Imaging 24, 959–962.
Louie, A. Y., Huber, M. M., Ahrens, E. T., Rothbacher, U., Moats, R., Jacobs, R. E., Fraser, S. E., and Meade, T. J. (2000) In vivo visualization of gene expression using magnetic resonance imaging. Nat Biotechnol 18, 321–325.
Goffeney, N., Bulte, J. W., Duyn, J., Bryant, L. H., Jr., and van Zijl, P. C. (2001) Sensitive NMR detection of cationic-polymer-based gene delivery systems using saturation transfer via proton exchange. J Am Chem Soc 123, 8628–8629.
McMahon, M., Gilad, A., Zhou, J., Sun, P., Bulte, J., and van Zijl, P. (2006) Quantifying exchange rates in chemical exchange saturation transfer agents using the saturation time and saturation power dependencies of the magnetization transfer effect on the magnetic resonance imaging signal (QUEST and QUESP): pH calibration for poly-L-lysine and a starburst dendrimer. Magn Reson Med 55.
Gilad, A., McMahon, M., Walczak, P., Winnard, P., Raman, V., van Laarhoven, H., Skoglund, C., Bulte, J., and van Zijl, P. (2007) Artificial reporter gene providing MRI contrast based on proton exchange. Nat Biotechnol 25, 217–219.
Gilad, A. A., Winnard, P. T., Jr., van Zijl, P. C., and Bulte, J. W. (2007) Developing MR reporter genes: promises and pitfalls. NMR Biomed 20, 275–290.
Gilad, A. A., Ziv, K., McMahon, M. T., van Zijl, P. C., Neeman, M., and Bulte, J. W. (2008) MRI Reporter Genes. J Nucl Med 12, 1905–1908.
Ward, K. M., Aletras, A. H., and Balaban, R. S. (2000) A new class of contrast agents for MRI based on proton chemical exchange dependent saturation transfer (CEST). J Magn Reson 143, 79–87.
Zhou, J., and van Zijl, P. C. (2006) Chemical exchange saturation transfer imaging and spectroscopy. Prog Nucl Magn Reson Spectrosc 48, 109–136.
Sherry, A. D., and Woods, M. (2008) Chemical exchange saturation transfer contrast agents for magnetic resonance imaging. Annu Rev Biomed Eng 10, 391–411.
McMahon, M. T., Gilad, A. A., DeLiso, M. A., Berman, S. M., Bulte, J. W., and van Zijl, P. C. (2008) New “multicolor” polypeptide diamagnetic chemical exchange saturation transfer (DIACEST) contrast agents for MRI. Magn Reson Med 60, 803–812.
Sun, P., Farrar, C., and Sorensen, A. (2007) Correction for artifacts induced by B0 and B1 field inhomogeneities in pH-sensitive chemical exchange saturation transfer (CEST) imaging. Magn Reson Med 58.
Kim, M., Gillen, J., Landman, B. A., Zhou, J., and van Zijl, P. C. M. (2009) WAter Saturation Shift Referencing (WASSR) for chemical exchange saturation transfer experiments. Mag Res Med 61, 1441–1450.
Zhou, J., Blakeley, J., Hua, J., Kim, M., Laterra, J., Pomper, M., and van Zijl, P. (2008) Practical data acquisition method for human brain tumor amide proton transfer (APT) imaging. Magn Reson Med 60.
Morrison, C., and Henkelman, R. M. (1995) A model for magnetization transfer in tissues. Magn Reson Med 33, 475–482.
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Liu, G., Gilad, A.A. (2011). MRI of CEST-Based Reporter Gene. In: Schröder, L., Faber, C. (eds) In vivo NMR Imaging. Methods in Molecular Biology, vol 771. Humana Press. https://doi.org/10.1007/978-1-61779-219-9_36
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DOI: https://doi.org/10.1007/978-1-61779-219-9_36
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