Abstract
Large insert genome libraries have been a core resource required to sequence genomes, analyze haplotypes, and aid gene discovery. While next generation sequencing technologies are revolutionizing the field of genomics, traditional genome libraries will still be required for accurate genome assembly. Their utility is also being extended to functional studies for understanding DNA regulatory elements. Here, we present a detailed method for constructing genomic fosmid libraries, testing for common contaminants, gridding the library to nylon membranes, then hybridizing the library membranes with a radiolabeled probe to identify corresponding genomic clones. While this chapter focuses on fosmid libraries, many of these steps can also be applied to bacterial artificial chromosome libraries.
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Quail, M.A., Matthews, L., Sims, S., Lloyd, C., Beasley, H., Baxter, S.W. (2012). Genomic Libraries: I. Construction and Screening of Fosmid Genomic Libraries. In: Orgogozo, V., Rockman, M. (eds) Molecular Methods for Evolutionary Genetics. Methods in Molecular Biology, vol 772. Humana Press. https://doi.org/10.1007/978-1-61779-228-1_3
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DOI: https://doi.org/10.1007/978-1-61779-228-1_3
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Publisher Name: Humana Press
Print ISBN: 978-1-61779-227-4
Online ISBN: 978-1-61779-228-1
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