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Adeno-Associated Virus Vector Delivery to the Heart

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Adeno-Associated Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 807))

Abstract

Cardiac gene transfer may serve as a novel therapeutic approach in the treatment of heart disease. For it to reach its full potential, methods for highly efficient cardiac gene transfer must be available to investigators so that informative preclinical data can be collected and evaluated. We have recently optimized AAV-mediated cardiac gene transfer protocols in both the mouse and rat. In the mouse, we have developed a procedure for intrapericardial delivery of vector in the neonate and successfully applied intravenous injections in adult animals. In the rat, we have developed a procedure for direct injection of vector into the myocardium in adults and established a protocol for vector delivery into the left ventricular anterior wall by ultrasound-targeted destruction of microbubbles loaded with AAV. Each protocol can be used to achieve safe and efficient cardiac gene transfer in the model of choice.

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Acknowledgments

This work was supported by a grant from the NHLBI (P01-HL059407 to HLS) and by TG-HL-007748 to LTB. Further support was obtained by a grant of the Deutsche Forschungsgemeinschaft (MU 1654/3-2) and the Bundesministerium für Bildung und Forschung (01GU0527) to OJM as well as by the BioFuture grant of the Bundesministerium für Bildung und Forschung to RB. We thank Stefanie Schinkel for her help in preparing this manuscript.

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Authors and Affiliations

  1. Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    Lawrence T. Bish & H. Lee Sweeney

  2. Internal Medicine, University of Heidelberg, Heidelberg, Germany

    Oliver J. Müller & Raffi Bekeredjian

Authors
  1. Lawrence T. Bish
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  2. H. Lee Sweeney
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  3. Oliver J. Müller
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  4. Raffi Bekeredjian
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Corresponding author

Correspondence to H. Lee Sweeney .

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Editors and Affiliations

  1. Center of Excellence for Regenerative, Health Biotechnology (CERHB), University of Florida, Innovation Drive 13706, Alachua, 32615, Florida, USA

    Richard O. Snyder

  2. INSERM U649, Labo. Thérapie Génique, CHU Hôtel Dieu, bd. Jean Monnet 30, Nantes CX 1, 44035, France

    Philippe Moullier

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Bish, L.T., Sweeney, H.L., Müller, O.J., Bekeredjian, R. (2012). Adeno-Associated Virus Vector Delivery to the Heart. In: Snyder, R., Moullier, P. (eds) Adeno-Associated Virus. Methods in Molecular Biology, vol 807. Humana Press. https://doi.org/10.1007/978-1-61779-370-7_9

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  • DOI: https://doi.org/10.1007/978-1-61779-370-7_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-369-1

  • Online ISBN: 978-1-61779-370-7

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