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Mass Spectrometry for Protein Quantification in Biomarker Discovery

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Functional Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 815))

Abstract

Major technological advances have made proteomics an extremely active field for biomarker discovery in recent years due primarily to the development of newer mass spectrometric technologies and the explosion in genomic and protein bioinformatics. This leads to an increased emphasis on larger scale, faster, and more efficient methods for detecting protein biomarkers in human tissues, cells, and biofluids. Most current proteomic methodologies for biomarker discovery, however, are not highly automated and are generally labor-intensive and expensive. More automation and improved software programs capable of handling a large amount of data are essential to reduce the cost of discovery and to increase throughput. In this chapter, we discuss and describe mass spectrometry-based proteomic methods for quantitative protein analysis.

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Acknowledgment

The authors would like to thank Ms. Heather Sahm for critical reading of this book chapter.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, MS 4053, Indianapolis, IN, 46202, USA

    Mu Wang & Jinsam You

Authors
  1. Mu Wang
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  2. Jinsam You
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Correspondence to Mu Wang .

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Editors and Affiliations

  1. School of Medicine, Witten/Herdecke University, Group Stockumer Str. 10 50, Witten, 58448, Germany

    Michael Kaufmann

  2. School of Medicine, Private Universität Witten/Herdecke, Witten/Herdecke University 50, Witten, 58448, Germany

    Claudia Klinger

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Wang, M., You, J. (2012). Mass Spectrometry for Protein Quantification in Biomarker Discovery. In: Kaufmann, M., Klinger, C. (eds) Functional Genomics. Methods in Molecular Biology, vol 815. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-424-7_16

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  • DOI: https://doi.org/10.1007/978-1-61779-424-7_16

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