Abstract
Female fertility is critically influenced by two events affecting oocytes during meiotic prophase progression: meiotic recombination between homologous chromosomes; and a major oocyte loss. It is technically challenging to examine these events, which take place in fetal and neonatal ovaries in mammals. Here, we describe a protocol for the preparation of dissociated ovarian cells and their spread onto histology slides. These preparations are suitable for cytogenetic, quantitative, and FISH analyses.
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Acknowledgements
We would like to acknowledge Drs. H. Merchant-Larios (University of Mexico) and P. Moens (deceased) for inspiring us to develop the methods described here. We also thank our previous graduate students, especially Kelly McClellan and Michele Alton, who contributed to developing and applying the described methods. We finally thank Dr. P. Burgoyne and the members of his division for allowing me to test the alternative methods described here.
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Taketo, T. (2012). Microspread Ovarian Cell Preparations for the Analysis of Meiotic Prophase Progression in Oocytes with Improved Recovery by Cytospin Centrifugation. In: Chan, WY., Blomberg, L. (eds) Germline Development. Methods in Molecular Biology, vol 825. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-436-0_13
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DOI: https://doi.org/10.1007/978-1-61779-436-0_13
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