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An Efficient Purification System for Native Minichromosome from Saccharomyces cerevisiae

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Chromatin Remodeling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 833))

Abstract

We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.

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References

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Acknowledgments

This work was supported in part by grants from NIGMS and Leukemia & Lymphoma Society to S.B. and T.T and a Beckman Young Investigator Award to S.B.

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Authors and Affiliations

  1. Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    Ashwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins & Toshio Tsukiyama

Authors
  1. Ashwin Unnikrishnan
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  2. Bungo Akiyoshi
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  3. Sue Biggins
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  4. Toshio Tsukiyama
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Corresponding author

Correspondence to Toshio Tsukiyama .

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Editors and Affiliations

  1. , Wadsworth Center, New York State Department of Health, New Scotland Avenue 120, Albany, 12208, New York, USA

    Randall H. Morse

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Unnikrishnan, A., Akiyoshi, B., Biggins, S., Tsukiyama, T. (2012). An Efficient Purification System for Native Minichromosome from Saccharomyces cerevisiae . In: Morse, R. (eds) Chromatin Remodeling. Methods in Molecular Biology, vol 833. Humana Press. https://doi.org/10.1007/978-1-61779-477-3_8

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  • DOI: https://doi.org/10.1007/978-1-61779-477-3_8

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-476-6

  • Online ISBN: 978-1-61779-477-3

  • eBook Packages: Springer Protocols

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