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Separation of Natural Products by Countercurrent Chromatography

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Natural Products Isolation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 864))

Abstract

Countercurrent Chromatography (CCC) provides the natural product chemist with a high-resolution separatory method, which is uniquely applicable to sensitive (unstable) compounds and which allows virtually quantitative recovery of the load sample. Different instruments use different means of retaining a stationary liquid phase. The solvent system (SS) can be chosen to optimize the separatory power and the number of systems available is limitless. Several examples are provided to illustrate the power of the method and to guide the chemist in choice of an appropriate SS.

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Authors and Affiliations

  1. Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA

    James B. McAlpine, J. Brent Friesen & Guido F. Pauli

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  1. James B. McAlpine
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  2. J. Brent Friesen
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  3. Guido F. Pauli
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Correspondence to James B. McAlpine .

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Editors and Affiliations

  1. School of Applied Sciences, Department of Pharmacy, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, United Kingdom

    Satyajit D. Sarker

  2. , Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, United Kingdom

    Lutfun Nahar

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McAlpine, J.B., Friesen, J.B., Pauli, G.F. (2012). Separation of Natural Products by Countercurrent Chromatography. In: Sarker, S., Nahar, L. (eds) Natural Products Isolation. Methods in Molecular Biology, vol 864. Humana Press. https://doi.org/10.1007/978-1-61779-624-1_9

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  • DOI: https://doi.org/10.1007/978-1-61779-624-1_9

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