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Scaffold Pore Space Modulation Through Intelligent Design of Dissolvable Microparticles

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Computer-Aided Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 868))

Abstract

The goal of this area of research is to manipulate the pore space of scaffolds through the application of an intelligent design concept on dissolvable microparticles. To accomplish this goal, we developed an efficient and repeatable process for fabrication of microparticles from multiple materials using a combination of rapid prototyping (RP) and soft lithography. Phase changed 3D printing was used to create masters for PDMS molds. A photocrosslinkable polymer was then delivered into these molds to make geometrically complex 3D microparticles. This repeatable process has demonstrated to generate the objects with greater than 95% repeatability with complete pattern transfer. This process was illustrated for three different shapes of various complexities. The shapes were based on the extrusion of 2D shapes. This may allow simplification of the fabrication process in the future combined with a direct transfer of the findings.

Altering the shapes of particles used for porous scaffold fabrication will allow for tailoring of the pore shapes, and therefore their biological function within a porous tissue engineering scaffold. Through permeation experiments, we have shown that the pore geometry may alter the permeability coefficient of scaffolds while influencing mechanical properties to a lesser extent. By selecting different porogen shapes, the nutrition transport and scaffold degradation can be significantly influenced with minimal effect on the mechanical integrity of the construct. In addition, the different shapes may allow a control of drug release by modifying their surface-to-volume ratio, which could modulate drug delivery over time. While soft lithography is currently used with photolithography, its high precision is offset by high cost of production. The employment of RP to a specific resolution offers a much less expensive alternative with increased throughput due to the speed of current RP systems.

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Authors and Affiliations

  1. Department of Neurosurgery, Baylor College of Medicine, Houston, TX, USA

    Michael A. K. Liebschner

  2. Research Service Line, Michael E. DeBakey VA Medical Center, Houston, TX, USA

    Michael A. K. Liebschner

  3. Department of Bioengineering, Caroline Collective, Houston, TX, USA

    Matthew Wettergreen

Authors
  1. Michael A. K. Liebschner
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  2. Matthew Wettergreen
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Correspondence to Michael A. K. Liebschner .

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Editors and Affiliations

  1. , Department of Neurosurgery, Baylor College of Medicine, Holcombe Blvd. 2002, Houston, 77030, Texas, USA

    Michael A.K. Liebschner

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Liebschner, M.A.K., Wettergreen, M. (2012). Scaffold Pore Space Modulation Through Intelligent Design of Dissolvable Microparticles. In: Liebschner, M. (eds) Computer-Aided Tissue Engineering. Methods in Molecular Biology, vol 868. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-764-4_5

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  • DOI: https://doi.org/10.1007/978-1-61779-764-4_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-763-7

  • Online ISBN: 978-1-61779-764-4

  • eBook Packages: Springer Protocols

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