Abstract
Human leukocyte antigen-E (HLA-E) is a non-classical HLA class I gene that shows a limited degree of polymorphism compared to the classical HLA genes. The HLA-E molecule can bind peptides derived from the leader sequence of various HLA class I alleles and some viral homologues, including CMV. The HLA-E peptide complex can act as a ligand for the CD94/NKG2 receptors expressed on the surface of natural killer cells and T cell subsets. Differences in expression levels between the different HLA-E alleles have been reported and a role for HLA-E polymorphism in stem cell transplantation has been postulated. This chapter focuses on routine technologies for HLA-E typing: the sequence-specific primer-PCR method that uses sequence-specific primers, the PCR sequence-specific oligonucleotides Luminex method, using sequence-specific probes attached to beads and the sequencing-based typing method, where sequencing of the alleles is performed.
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Acknowledgements
The authors thank Jarhow Lee (One Lambda, Los Angeles, USA) for the development of the HLA-E SSO kit and reagents and Els Bielen and Timo Olieslagers for their practical assistance.
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Lauterbach, N., Voorter, C.E.M., Tilanus, M.G.J. (2012). Molecular Typing of HLA-E. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_8
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DOI: https://doi.org/10.1007/978-1-61779-842-9_8
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