Abstract
The carotene producer fungus Mucor circinelloides is the zygomycete more amenable to genetic manipulations by using molecular tools. Since the initial development of an effective procedure of genetic transformation, more than two decades ago, the availability of new molecular approaches such as gene replacement techniques and gene expression inactivation by RNA silencing, in addition to the sequencing of its genome, has made Mucor a valuable organism for the study of a number of processes. Here we describe in detail the main techniques and methods currently used to manipulate M. circinelloides, including transformation, gene replacement, gene silencing, RNAi, and immunoprecipitation.
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Acknowledgments
The work in our laboratory is supplied by Fundacion Séneca de la Comunidad Autónoma de la Región de Murcia, Spain (project 08802/PI/08), by D.G. de Investigación y Política Científica (Comunidad Autónoma de la Región de Murcia, Spain; Project Bio-BMC 07/01-0005), and Spanish Ministerio de Ciencia e Innovación (BFU2006-02408, BFU2009-07220).
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Torres-Martínez, S., Ruiz-Vázquez, R.M., Garre, V., López-García, S., Navarro, E., Vila, A. (2012). Molecular Tools for Carotenogenesis Analysis in the Zygomycete Mucor circinelloides . In: Barredo, JL. (eds) Microbial Carotenoids From Fungi. Methods in Molecular Biology, vol 898. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-918-1_5
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DOI: https://doi.org/10.1007/978-1-61779-918-1_5
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