Abstract
Immunoaffinity capillary electrophoresis (ICE) is a powerful tool used to detect and quantify target proteins of interest in complex biological fluids. The target analyte is captured and bound to antibodies immobilized onto the wall of a capillary, labeled in situ with a fluorescent dye, eluted and detected online using laser-induced fluorescence following electrophoretic separation. Here, we illustrate how to construct an immunoaffinity capillary and utilize it to run ICE in order to capture and quantify target cytokines and chemokines from a clinical sample.
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Mendonca, M., Kalish, H. (2013). Cytokine Analysis by Immunoaffinity Capillary Electrophoresis. In: Phillips, T., Kalish, H. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 919. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-029-8_23
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DOI: https://doi.org/10.1007/978-1-62703-029-8_23
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-028-1
Online ISBN: 978-1-62703-029-8
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