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Capillary Electrophoresis with Laser-Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids

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Capillary Electrophoresis of Biomolecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 984))

Abstract

Sample preparation and laser-induced fluorescence detection are two key steps of the analytical methodology to determine by capillary electrophoresis low concentrations of proteins in complex sample matrices. In this chapter the options of performing both steps in different ways are shown by detailing the analysis of the allergen β-lactoglobulin in food products for infants and the analysis of the isoforms of alpha 1-acid glycoprotein, a potential biomarker, in serum and secretome.

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Acknowledgments

Isabel Vasallo from Hero Institute for Infant Nutrition (Hero España, S.A., Murcia, Spain) is acknowledged for preparing the infant food samples. Financial support from the Spanish Ministry of Science and Innovation (project CTQ2009-09399), CSIC (project 2010JP0013), and the Hungarian Government (project OTKA K-81839) is acknowledged. Angel Puerta acknowledges CSIC for a contract in the JAE doc program cofinanced by the European Union under the European Social Fund, and Raul Garrido-Medina acknowledges the Spanish Ministry of Education the Ph.D. fellowship (FPU) awarded.

Author information

Authors and Affiliations

  1. Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain

    Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo & Jose Carlos Diez-Masa

  2. European University of Madrid, Madrid, Spain

    Cristina Pelaez-Lorenzo

  3. Horváth Laboratory of Bioseparation Sciences, University of Debrecen, Debrecen, Hungary

    Zuly Rivera-Monroy & Andras Guttman

  4. Hungary National University of Colombia, Bogota, Colombia

    Zuly Rivera-Monroy

  5. Institute of Organic Chemistry (IQOG, CSIC), Madrid, Spain

    Mercedes de Frutos

Authors
  1. Raul Garrido-Medina
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  2. Angel Puerta
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  3. Cristina Pelaez-Lorenzo
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  4. Zuly Rivera-Monroy
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  5. Andras Guttman
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  6. Jose Carlos Diez-Masa
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  7. Mercedes de Frutos
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Corresponding author

Correspondence to Mercedes de Frutos .

Editor information

Editors and Affiliations

  1. Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213/D, Modena, 41125, Italy

    Nicola Volpi

  2. Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213/D, Modena, 41125, Italy

    Francesca Maccari

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Garrido-Medina, R. et al. (2013). Capillary Electrophoresis with Laser-Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_15

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  • DOI: https://doi.org/10.1007/978-1-62703-296-4_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-295-7

  • Online ISBN: 978-1-62703-296-4

  • eBook Packages: Springer Protocols

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