Abstract
Capillary electrophoresis (CE) is one among a number of highly sensitive chemical separation techniques used to characterize single or a small number of cells and to develop assays of enzymatic activity. Other commonly used techniques include mass spectrometry and electrochemistry; however, CE using laser-induced fluorescence detection (LIF) is the most sensitive of these techniques. In CE-LIF, fluorescently labeled proteins or lipids are normally separated based on their size to charge ratio in the interior of a small capillary filled with an electrolyte upon the application of an electric field. In this chapter, we describe the application of CE-LIF for the determination of the bioactivity of fluorescently lipids and sphingosine kinase activity.
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Acknowledgment
This research study was supported by the Kent State University research startup fund and the Farris Innovation award.
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Yangyuoru, P.M., Hammonds-Odie, L., Mwongela, S.M. (2013). Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_24
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DOI: https://doi.org/10.1007/978-1-62703-296-4_24
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