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Lentiviral-Mediated Gene Transfer of siRNAs for the Treatment of Huntington’s Disease

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Trinucleotide Repeat Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1010))

Abstract

This chapter describes the potential use of viral-mediated gene transfer in the central nervous system for the silencing of gene expression using RNA interference in the context of Huntington’s disease (HD). Protocols provided here describe the design of small interfering RNAs, their encoding in lentiviral vectors (LVs) and viral production, as well as procedures for their stereotaxic injection in the rodent brain.

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Author information

Authors and Affiliations

  1. Molecular Imaging Research Center, Commissariat à l’Énergie Atomique, Fontenay-aux-Roses, France

    Karine Cambon

  2. Département des Neurosciences Cliniques (DNC), Centre Hospitalier Universitaire Vaudois (CHUV), Lausanne, Switzerland

    Nicole Déglon

Authors
  1. Karine Cambon
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  2. Nicole Déglon
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Editor information

Editors and Affiliations

  1. Life Sciences Division, Lawrence Berkeley National Laboratory, Cyclotron Road, MS-977 1, Berkeley, 94720, California, USA

    Yoshinori Kohwi

  2. Life Sciences Division, Lawrence Berkeley National Laboratory, Cyclotron Road, MS-83 1, Berkeley, 94720, California, USA

    Cynthia T. McMurray

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© 2013 Springer New York

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Cambon, K., Déglon, N. (2013). Lentiviral-Mediated Gene Transfer of siRNAs for the Treatment of Huntington’s Disease. In: Kohwi, Y., McMurray, C. (eds) Trinucleotide Repeat Protocols. Methods in Molecular Biology, vol 1010. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-411-1_7

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  • DOI: https://doi.org/10.1007/978-1-62703-411-1_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-410-4

  • Online ISBN: 978-1-62703-411-1

  • eBook Packages: Springer Protocols

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