Abstract
Uveitis is a common cause of vision loss, accounting for 10–15 % of all cases of blindness worldwide and affects individuals of all ages, genders, and races. Uveitis represents a broad range of intraocular inflammatory conditions due to complications of autoimmune diseases, bacterial infections, viral infections, and chemical and metabolic injuries. Endotoxin-induced uveitis (EIU) in rodents is an efficient experimental model to investigate the pathological mechanism and pharmacological efficacy of potential drug agents. EIU is characterized by clinically relevant classical signs of inflammation, including inflammatory exudates and cells in the anterior and vitreous chambers. EIU in small animal models such as rats, mice, and rabbits is a short-lived uveal inflammation that can be developed subsequent to administration of bacterial endotoxin, such as lipopolysaccharide. Here, we present a reproducible, reliable, and simplified protocol to induce EIU in mice. This method could be used with similar efficacy for EIU induction in other small animals as well.
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References
de Smet MD, Taylor SR, Bodaghi B et al (2011) Understanding uveitis: the impact of research on visual outcomes. Prog Retin Eye Res 30:452–470
Rathinam SR, Namperumalsamy P (2007) Global variation and pattern changes in epidemiology of uveitis. Indian J Ophthalmol 55:173–183
Nussenblatt RB (1990) The natural history of uveitis. Int Ophthalmol 14:303–308
Read R (2006) Uveitis: advances in understanding of pathogenesis. CurrRheumatol Rep 8:260–266
Gritz DC, Wong IG (2004) Incidence and prevalence of uveitis in Northern California; the Northern California epidemiology of uveitis study. Ophthalmology 111:491–500
Rosenbaum JT, McDevitt HO, Guss RB et al (1980) Endotoxin induced uveitis in rats as a model for human disease. Nature 286:611–613
Chatzistefanou K, Markomichelakis NN, Christen W et al (1998) Characteristics of uveitis presenting for the first time in the elderly. Ophthalmology 105:347–352
Yadav UC, Kalariya NM, Ramana KV (2011) Emerging role of antioxidants in the protection of uveitis complications. Curr Med Chem 18:931–942
Altan-Yaycioglu R, Akova YA, Akca S et al (2006) Inflammation of the posterior uvea: findings on fundus fluorescein and indocyanine green angiography. Ocul Immunol Inflamm 14:171–179
Yadav UC, Srivastava SK, Ramana KV (2007) Aldose reductase inhibition prevents endotoxin-induced uveitis in rats. Invest Ophthalmol Vis Sci 48:4634–4642
Kamala T (2007) Hock immunization: a humane alternative to mouse footpad injections. J Immunol Methods 328:204–214
Shen DF, Chang MA, Matteson DM, Buggage R, Kozhich AT, Chan CC (2000) Biphasic ocular inflammatory response to endotoxin-induced uveitis in the mouse. Arch Ophthalmol 118:521–527
Yadav UC, Shoeb M, Srivastava SK, Ramana KV (2011) Aldose reductase deficiency protects from autoimmune- and endotoxin-induced uveitis in mice. Invest Ophthalmol Vis Sci 52:8076–8085
Acknowledgments
This work in the author’s lab was supported by National Institutes of Health (NIH) Grant EY015891 to K.V.R.
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Yadav, U.C.S., Ramana, K.V. (2013). Endotoxin-Induced Uveitis in Rodents. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_18
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DOI: https://doi.org/10.1007/978-1-62703-481-4_18
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-481-4
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