Abstract
Extracellular vesicles (EVs) including exosomes and microvesicles are nanometer-sized vesicles released by cells to deliver lipids, cellular proteins, mRNAs, and noncoding RNAs, thereby facilitating intercellular communication without direct cell-to-cell contacts. Due to their nanoscale size, EVs have been visualized under microscopy in vitro. We here describe a strategy to label EVs with Gaussia luciferase for noninvasive bioluminescence imaging and monitoring of systemically administered EVs in vivo.
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Acknowledgement
We wish to thank Mr. Osama Mardini for his technical assistance. This work was supported by NIH grant NIH/NCI CA069246, CA141150 and Canadian Institutes of Health Research (CIHR).
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Lai, C.P., Tannous, B.A., Breakefield, X.O. (2014). Noninvasive In Vivo Monitoring of Extracellular Vesicles. In: Badr, C. (eds) Bioluminescent Imaging. Methods in Molecular Biology, vol 1098. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-718-1_19
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DOI: https://doi.org/10.1007/978-1-62703-718-1_19
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