Abstract
Aptamer technology allows for the selection of nucleic acids that can bind to and enter cells. By establishing conditions during the selection that eliminate cell-surface binders as well as non-internalizing RNAs, only extremely tightly bound aptamers or aptamers that have internalized are recovered. We describe a general scheme for selecting RNA molecules that are capable of internalizing into cells and discuss the factors that can affect a successful selection. Much like standard cell-surface selections, these types of selections should be possible independent of detailed knowledge of the cell surface.
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Acknowledgement
This work was supported by a grant from Stand Up 2 Cancer (SU2C-AACR-IRG0809).
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Yan, A., Levy, M. (2014). Cell Internalization SELEX: In Vitro Selection for Molecules That Internalize into Cells. In: Lafontaine, D., Dubé, A. (eds) Therapeutic Applications of Ribozymes and Riboswitches. Methods in Molecular Biology, vol 1103. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-730-3_18
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DOI: https://doi.org/10.1007/978-1-62703-730-3_18
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Publisher Name: Humana Press, Totowa, NJ
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