Abstract
In immunoelectron microscopy (IEM), simultaneous labeling of two or more antigens on the same section is desirable for many applications. If the antibodies (Ab) to be used are raised in the same species, as is usually the case with monoclonal antibodies (MAb), the difficulty arises that the labeled secondary, antispecies Ab used in the first labeling step traps the primary Ab directed against the second antigen, thus leading to a nonspecific signal for the second antigen.
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References
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© 1992 The Humana Press, Inc., Totowa, NJ
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Bienz, K., Egger, D. (1992). Electron Microscopic Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species. In: Manson, M.M. (eds) Immunochemical Protocols. Methods in Molecular Biology, vol 10. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-204-3:187
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DOI: https://doi.org/10.1385/0-89603-204-3:187
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-204-0
Online ISBN: 978-1-59259-497-9
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