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Collection and Processing of Mucosal Secretions from Mice

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HIV Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 17))

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Abstract

The major route of transmission of the human immunodeficiency virus type 1 (HIV-1) worldwide, like other agents of sexually transmitted disease, is via mucosal surfaces of the genital tract through sexual exposures (1–4). It has been hypothesized that immune responses at these sites may be important determinants of protection against HIV-1 (1,2,5). Development of HIV-1 vaccines which elicit local mucosal immune responses may be critical for an effective preventive vaccine. Specific mucosal immune responses elicited after different routes of mucosal immunization in rodents have been examined previously (6–16). Nasal and oral immunization routes have elicited strong mucosal responses to HIV-1 peptides and whole proteins in mice (7,11,17,18,24). Induction of mucosal and systemic immunity to SIV and HIV-1 antigens in primates has also been obtained using a variety of mucosal routes of vaccine administration (19–22).

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References

  1. Mestecky, J. and Jackson, S. (1994) Reassessment of the impact of mucosal immunity in infection with the human immunodeficiency virus (HIV) and design of relevant vaccines. J. Clin. Immunol. 14, 259–272.

    Article  PubMed  CAS  Google Scholar 

  2. Miller, C. J., McGhee, J. R., and Gardner, M. B. (1993) Mucosal immunity, HIV transmission, and AIDS. Lab. Invest. 68, 129–145.

    PubMed  CAS  Google Scholar 

  3. Piot, P., Plummer, F. A., Mhalu, F. S., Lamboray, J. L., Chin, J., and Mann, J. M. (1988) AIDS: an international perspective. Science 239, 573–579.

    Article  PubMed  CAS  Google Scholar 

  4. Padian, N. S., Shiboski, S. C., and Jewell, N. P. (1991) Female-to-male transmission of human immunodeficiency virus. JAMA 266, 1664–1667.

    Article  PubMed  CAS  Google Scholar 

  5. Forrest, B.D. (1991) Women, HIV, and mucosal immunity. Lancet 337, 835,836.

    Article  PubMed  CAS  Google Scholar 

  6. Haneberg, B., Kendall, D., Amerongen, H. M., Apter, F. M., Kraehenbuhl, J. P., and Neutra, M. R. (1994) Induction of specific immunoglobulin A in the small intestine, colon-rectum, and vagina measured by a new method for collection of secretions from local mucosal surfaces. Infect. Immunol. 62, 15–23.

    CAS  Google Scholar 

  7. Muster, T., Ferko, B., Klima, A., Purtscher, M., Trkola, A., Schulz, P., et al. (1995) Mucosal model of immunization against human immunodeficiency virus type 1 with a chimeric influenza virus. J. Virol. 69, 6678–6686.

    PubMed  CAS  Google Scholar 

  8. O’Hagan, D. T., Rafferty, D., McKeating, J. A., and Ilium, L. (1992) Vaginal immunization of rats with a synthetic peptide from human immunodeficiency virus envelope glycoprotein. J. Gen. Virol. 73, 2141–2145.

    Article  PubMed  Google Scholar 

  9. O’Hagan, D. T., McGee, J. P., Holmgren, J., Mowat, A. M., Donachie, A. M., Mills, et al. (1993) Biodegradable microparticles for oral immunization. Vaccine 11,149–154.

    Article  PubMed  Google Scholar 

  10. Orr, N., Robin, G., Cohen, D., Arnon, R., and Lowell, G. H. (1993) Immunogenicity and efficacy of oral or intranasal Shigella flexneri 2a and Shigella sonnei proteosome-lipopolysaccharide vaccines in animal models. Infect. Immunol. 61, 2390–2395.

    CAS  Google Scholar 

  11. Staats, H. F., Nichols, W. G., and Palker, T. J. (1996) Mucosal immunity to HIV-1: systemic and vaginal antibody responses after intranasal immunization with the HIV-1 C4/V3 peptide T1SP10 MN(A). J. Immunol. 157, 464–472.

    Google Scholar 

  12. Abraham, E. and Shah, S. (1992) Intranasal immunization with liposomes containing IL-2 enhances bacterial polysaccharide antigen-specific pulmonary secretory antibody response. J. Immunol. 149, 3719–3726.

    PubMed  CAS  Google Scholar 

  13. Vajdy, M. and Lycke, N. Y. (1992) Cholera toxin adjuvant promotes long-term immunological memory in the gut mucosa to unrelated immunogens after oral immunization. Immunology 75, 488–492.

    PubMed  CAS  Google Scholar 

  14. Langermann, S., Palaszynski, S., Sadziene, A., Stover, C. K., and Koenig, S. (1994) Systemic and mucosal immunity induced by BCG vector expressing outer-surface protein A of Borrelia burgdorferi. Nature 372, 552–555.

    Article  PubMed  CAS  Google Scholar 

  15. Vadolas, J., Davies, J. K., Wright, P. J., and Strugnell, R. A. (1995) Intranasal immunization with liposomes induces strong mucosal immune responses in mice. Eur. J. Immunol. 25, 969–975.

    Article  PubMed  CAS  Google Scholar 

  16. VanCott, J. L., Staats, H. F., Pascual, D. W., Roberts, M., Chatfield, S. N., Yamamoto, M., et al. (1996) Regulation of mucosal and systemic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella. J. Immunol. 156, 1504–1514.

    PubMed  CAS  Google Scholar 

  17. Bukawa, H., Sekigawa, K., Hamajima, K., Fukushima, J., Yamada, Y., Kiyono, H., et al. (1995) Neutralization of HTV-1 by secretory IgA induced by oral immunization with a new macromolecular multicomponent peptide vaccine candidate. Nat. Med. 1, 681–685.

    Article  PubMed  CAS  Google Scholar 

  18. Lowell, G. H., Kaminski, R. W., VanCott, T. C, Slike, B., Kersey, K., Zawoznik, et al. (1997) Nasal immunization with HIV gpl60 plus proteosomes, emulsomes and/or cholera toxin B subunit to induce serum, intestinal, lung and vaginal antibodies. J. Infect. Dis. 175, 292–301.

    PubMed  CAS  Google Scholar 

  19. Lehner, T., Bergmeier, L. A., Panagiotidi, C., Tao, L., Brookes, R., Klavinskis, et al. (1992) Induction of mucosal and systemic immunity to a recombinant simian immunodeficiency viral protein. Science 258, 1365–1369.

    Article  PubMed  CAS  Google Scholar 

  20. Lehner, T., Bergmeier, L.A., Tao, L., Panagiotidi, C., Klavinskis, L.S., Hussain, L., et al. (1994) Targeted lymph node immunization with simian immunodeficiency virus p27 antigen to elicit genital, rectal, and urinary immune responses in nonhuman primates. J. Immunol. 153, 1858–1868.

    PubMed  CAS  Google Scholar 

  21. Marx, P. A., Compans, R. W., Gettie, A., Staas, J. K., Gilley, R. M., Mulligan, M. J., et al. (1993) Protection against vaginal SIV transmission with microencapsulated vaccine. Science 260, 1323–1327.

    Article  PubMed  CAS  Google Scholar 

  22. Lubeck, M. D., Natuk, R. J., Chengalvala, M., Chanda, P. K., Murthy, K. K., Murthy, S., et al. (1994) Immunogenicity of recombinant adenovirus-human immunodeficiency virus vaccines in chimpanzees following intranasal administration. AIDS Res. Hum. Retrovir. 10, 1443–1449.

    Article  PubMed  CAS  Google Scholar 

  23. Orr, N., Arnon, R., Rubin, G., Cohen, D., Bercovier, H., and Lowell, G. H. (1994) Enhancement of anti-Shigella lipopolysaccharide (LPS) response by addition of the cholera toxin B subunit to oral and intranasal proteosome-Shigella flexneri 2a LPS vaccines. Infect. Immunol. 62, 5198–5200.

    CAS  Google Scholar 

  24. VanCott, T. C., Kaminski, R. W., Mascola, J. R. Kalyanaraman, V., Wassef, N., Alving, C., et al. (1998) HIV-1 neutralizing antibodies in the genital and respiratory tracts of mice intranasally immunized with oligomeric gp160. J. Immunol. 160, 2000–2012.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Henry M. Jackson Foundation, Rockville, MD

    Robert W. Kaminski & Thomas C. VanCott

Authors
  1. Robert W. Kaminski
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  2. Thomas C. VanCott
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Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Rockville, MD

    Nelson L. Michael MD, PhD

  2. Henry M. Jackson Foundation, Rockville, MD

    Jerome H. Kim MD

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© 1999 Humana Press Inc., Totowa, NJ

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Kaminski, R.W., VanCott, T.C. (1999). Collection and Processing of Mucosal Secretions from Mice. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicine™, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:329

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  • DOI: https://doi.org/10.1385/0-89603-369-4:329

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-369-6

  • Online ISBN: 978-1-59259-601-0

  • eBook Packages: Springer Protocols

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