Abstract
Cytotoxic T-cells (CTL) provide the basis of protective immunity in many viral infections by appearing early in the immune response and becoming involved with the elimination of virus by lysis of infected cells. A considerable amount of evidence suggests that CTL may be an especially important component of the host defense against HIV-1 (1,2) . Strong CTL activity is seen early during the course of infection (3,4), in nonprogressor individuals, some of whom have been infected for a decade or more with HIV-1 (5,6) and also in some HIV-1 exposed sex workers (7) and babies (8,9) who remain HIV-1-negative.
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References
Rowland-Jones, S. and McMichael, A. (1993) Cytotoxic T lymphocytes in HIV infection. Semin. Virol. 4, 83ā94.
Lederman, M. M. (1996) Role of cytotoxic T lymphocytes in the control of HIV-1 infection and HIV-1 disease progression. Cur. Opin. Infect. Dis. 9, 14ā18.
Koup, R. A., Safrit, J. T., Cao, Y., Andrews, C. A., McLeod, G., Borkwosky, W., Farthing, C., and Ho, D. D. (1994) Temporal association of cellular immune responses with the initial viremia in primary human immunodeficiency virus type 1 syndrome. J. Virol. 68, 4650ā4655.
Borrow, P., Lewicki, H., Hahn, B. H., Shaw, G. M., and Oldstone, M. B. A. (1994) Virus-specific CD8+ cytotoxic T lymphocyte activity associated with control of viremia in primary human immunodeficiency virus type 1 infection. J. Virol. 68,6103ā6110.
Harrer, T., Harrer, E., Kalams, S. A., Elbeik, T., Staprans, S. I., Feinberg, M. B., et al. (1996) Strong cytotoxic T cell and weak neutralizing antibody responses in a subset of persons with stable nonprogressing HIV Type 1 infection. AIDS Res. Hum. Retrovir. 12, 585ā592.
Klein, M. R., van Baalen, C. A., Holwerda, A. M., Kerkhof Garde, S. R., Bende, R. J., Keet, I. P., et al. (1995) Kinetics of Gag-specific cytotoxic T lymphocyte responses during the clinical course of HIV-1 infection: a longitudinal analysis of rapid progressors and long term asymptomatics. J. Exp. Med. 181, 1365ā1372.
Rowland-Jones, S., Sutton, J., Ariyoshi, K., Dong, T., Gotch, F., McAdam, S., et al. (1995) HIV-specific cytotoxic T-cells in HIV-exposed but uninfected Gambian women. Nat. Med. 1, 59ā64.
Cheynier, R., Langlade-Demoyen, P., Marescot, M. R., Blanche, S., Blondin, G., Wain-Hobson, S., et al. (1992) Cytotoxic T lymphocyte responses in the peripheral blood of children born to human immunodeficiency virus-1 infected mothers. Eur. J. Immunol. 2, 2211ā2217.
Rowland-Jones, S., Nixon, D. F., Aldhous, M. C., Gotch, F., Ariyoshi, K., Hallam, N., et al. (1993) HIV-specific cytotoxic T-cell activity in an HIV-exposed but uninfected infant. Lancet 341, 860ā861.
Walker, B. D., Chakrabarti, S., Moss, B., Paradis, T. J., Flynn, T., Durno, A. G., et al. (1987) HIV-specific cytotoxic T lymphocytes in seropositive individuals. Nature 328, 345ā348.
Hoffenbach, A., Langlade-Demoyen, P., Dadaglio, G., Vilmer, E., Michel, F., Mayaud, C, et al. (1989) Unusually high frequencies of HIV-specific cytotoxic T lymphocytes in humans. J. Immunol. 142,452ā462.
Koenig, S., Earl, P., Powell, B., Pantaleo, G., Merli, S., Moss, B., et al. (1988) Group-specific, major histocompatibility complex class I-restricted cytotoxic responses to human immunodeficiency virus I (HIV-1) envelope proteins by cloned peripheral blood T cells from an HIV-1-infected individual. Proc. Natl. Acad. Sci. USA 85, 8638ā8642.
Riviere, Y., Tanneau-Salvadori, F., Regnault, A., Lopez, O., Sansonetti, P., Guy, B., et al. (1989) Human immunodeficiency virus-specific cytotoxic responses of seropositive individuals: distinct types of effector cells mediate killing of targets expressing gag and env proteins. J. Virol. 63, 2270ā2277.
Blazevic, V., Ranki, A., and Krohn, K. J. E. (1995) Helper and cytotoxic T cell responses of HIV Type-1 infected individuals to synthetic peptides of REV. AIDS Res. and Hum. Retrovir. 11, 1335ā1342.
Nixon, D. F. and McMichael, A. J. (1991) Cytotoxic T-cell recognition of HIV proteins and peptides. AIDS 5, 1049ā1059.
Hammond, S. A., Bollinger, R. C., Stanhope, P. E., Quinn, T. C., Schwartz, D., Clements, M. L., and Siliciano, R. F. (1992) Comparative clonal analysis of human immunodeficiency virus type-1 (HIV-l)-specific CD4+ and CD8+ cytolytic T lymphocytes isolated from seronegative humans immunized with candidate HIV-1 vaccines. J. Exp. Med. 176, 1531ā1542.
Curiel, T. J., Wong, J. T., Gorczyca, P. F., Schooley, R. T., Walker, B. D., et al. (1993) CD4+ human immunodeficiency virus type-1 (HIV-1) envelope-specific cytotoxic T lymphocytes derived from the peripheral blood of an HIV-infected individual. AIDS Res. Hum. Retrovir. 9, 61ā68.
Ratto, S., Sitz, K. V., Scherer, A., Loomis, L. D., Cox, J. H., Redfield, R. R., and Birx, D. L. (1996) CD4+ T-lymphocyte lines developed from HIV-1 seropositive patients recognize difference epitopes within the V3 loop. J. AIDS 11, 128ā136.
Earl, P. L. and Moss, B. (1992) Expression of proteins in mammalian cells using vaccinia. Current Protocols In Molecular Biology, vol 2 (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., eds.) units 15.1-18.9.
van Baalen, C. A., Klein, M. R., Geretti, A. M., Keet, R. I., Miedema, F., van Els, C. A., and Osterhaus, A. D. (1993) Selective in vitro expansion of HLA Class I-restricted HIV-1 gag-specific CD8+ T cells: cytotoxic T-lymphocyte epitopes and precursor rrequencies. AIDS 7, 781ā786.
Goteh, F., McAdam, S. N., Allsopp, C. E. M., Gallimore, A., Elvin, J., Kieny, M. P., et al. (1993) Cytotoxic T cells in HTV-2 seropositive Gambians. J. Immunol. 151,3361ā3369.
McElrath, M., Siliciano, R. F., and Weinhold, K. J. (1997) HIV type 1 vaccine-induced cytotoxic T cell responses in phase I clinical trials: detection, characterization and quantitation. AIDS Res. Hum. Retrovir. 13, 211ā216.
Ferrari, G., Humphrey, W., McElrath, M.J., Excler, J. L., Duliege, A. M., Clements, M. L., et al. (1997). Clade B-based HIV-1 vaccines elicit cross-clade cytotoxic T lymphocyte reactivities in uninfected volunteers. P.N.A.S. 94, 1396ā1401.
Carmichael, A., Alp, N., and Borysiewicz, L. (1992) Quantitating the HIV-1 specific cytotoxic T cell response, in Cytotoxic T Cells in HIV and Other Retroviral Infections (Racz, P., Letvin N. L., and Gluckman, J. C., eds.), S. Karger, Basel, pp. 30ā39.
Fazekas de St Groth, S. (1982) The evaluation of limiting dilution analysis. J. Immunol. Methods 49, R11āR23.
Strijbosch, L. W. J., Does, R. J. M. M., and Buurman, W. A. (1988) Computer aided design and evaluation of limiting and serial dilution experiments. Int. J. Biomed. Comput. 23, 279ā290.
Geretti, A. M., Dings, M. E., van Els, C. A. van Baalen, C. A., Wijnholds, F. J., Borleffs, J. C., and Osterhaus, A. D. (1996) Human immunodeficiency virus Type 1 (HIV-1)-and Epstein-Barr Virus specific cytotoxic T lymphocyte precursors exhibit different kinetics in HIV-1 infected persons. J. Infect. Dis. 174, 34ā45.
Carini, C. and Essex, M. (1994) Interleukin 2-dependent interleukin 7 activity enhances cytotoxic immune response of HIV-1-infected individuals. AIDS Res. Hum. Retrovir. 10, 121ā130.
Ferrari, G., King, K., Rathbun, K., Place, C. A., Packard, M. V., Bartlett, J. A., et al. (1995) IL-7 enhancement of antigen-driven activation/expansion of HIV-1-specific cytotoxic T lymphocyte precursors (CTLp) Clin. Exp. Immunol. 101, 239ā248.
Blomberg, K., Granberg, C., HemmilƤ, I., and Lƶvgren, T. (1986) Europium-labeled target cells in an assay of natural killer cell activity. I. A novel non-radioactive method based on time-resolved fluorescence. J. Immunol. Methods 86, 225ā233.
Blomberg, K., Hautala, R., Lovgren, J., Mukkala, V. M., Lindqvist, C., and Akerman, K. (1996) Time resolved fluorimetric assay for natural killer activity using target cells labeled with a fluorescence enhancing ligand. J. Immunol. Methods 193, 199ā206.
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Cox, J.H. (1999). HIV-1-Specific Cytotoxic T-Cell Assays. In: Michael, N.L., Kim, J.H. (eds) HIV Protocols. Methods in Molecular Medicineā¢, vol 17. Humana Press. https://doi.org/10.1385/0-89603-369-4:355
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DOI: https://doi.org/10.1385/0-89603-369-4:355
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