Abstract
Immobilization of proteins on intact red blood cells (RBCs) can be valuable for several different reasons. In vivo, RBCs carrying specific ligands can be targeted to cells exposing specific antigens, eventually for the delivery of drugs encapsulated in the same RBC (1). Alternatively, RBCs carrying antigens or enzymes on the extracellular surface of their membrane can perform as antigen delivery systems (2) or as bioreactors for the degradation of toxic metabolites (3). In vitro, RBCs carrying proteins can be used as diagnostic reagents in agglutination or hemolytic assays (4).
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References
Chiarantini, L., Droleskey, R., Magnani, M., Kirch, H., and DeLoach, J. R. (1992) Targeting of erythrocytes to cytotoxic T-cells, in The Use of Resealed Erythrocytes as Carrier and Bioreactors (Magnani, M. and DeLoach, J. R., eds.), Plenum, New York, pp. 257–267.
Magnani, M., Chiarantini, L., Vittoria, E., Mancini, U., Rossi, L., and Fazi, A. (1992) Red blood cells as an antigen-delivery system. Biotechnol Appl. Biochem. 16, 188–194.
Magnani, M., Mancini, U., Bianchi, M., and Fazi, A. (1992) Comparison of uricase bound and uricase-loaded erythrocytes as bioreactors for uric acid degradation, in The Use of Reseated Erythrocytes as Carriers and Bioreactors (Magnani, M. and DeLoach, J. R., eds.), Plenum, New York, pp. 189–194.
Muzykantov, V. R., Samokhin, G. P., Smirnov, M. D., and Domogatosky, S. P. (1995) Hemolytic complement activity assay in micro titration plates. J. Appl. Biochem. 7, 223–227.
Gold, E. R. and Fudenberg, H. H. (1967) Chromic chloride: a coupling reagent for passive hemagglutination reactions. J. Immunol. 99, 859–865.
Jon, Y. H. and Bankert, R. B. (1981) Coupling of protein antigens to erythrocytes through disulphide bond formation: preparation of stable and sensitive target cells for immune hemolysis. Proc. Natl Acad. Scl. USA 78, 2493–2496.
Mouneimne, Y., Tosi, P. R., Barhoulmi, R., and Nicolau, C. (1991) Electroinsertion of xeno proteins in red blood cell membranes yield a long lived protein carrier in circulation. Biochim. Biophys. Acta 1066, 83–39.
Samokhin, G. P., Smirnov, M. D., Muzykantov, V. R., Domogatsky, S. P., and Smirnov, V. N. (1983) Red blood cell targeting to collagen-coated surfaces. FEBS Lett. 154, 257–261.
Magnani, M., Chiarantini, L., and Mancini, U. (1994) Preparation of characterization of biotinylated red blood cells. Biotechnol. Appl. Biochem. 20, 335–345.
O’Shannessy, D. J., Vaarstad, P. J., and Quarles, R. H. (1987) Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Anal. Biochem. 163, 204–209.
Suzuki, T. and Dale, G. L. (1987) Biotinytated erythrocytes: in vivo survival and in vitro recovery. Blood 70, 791–795.
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© 1997 Humana Press Inc , Totowa, NJ
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Chiarantini, L., Magnani, M. (1997). Immobilization of Enzymes and Proteins on Red Blood Cells. In: Bickerstaff, G.F. (eds) Immobilization of Enzymes and Cells. Methods in Biotechnology, vol 1. Humana Press. https://doi.org/10.1385/0-89603-386-4:143
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DOI: https://doi.org/10.1385/0-89603-386-4:143
Publisher Name: Humana Press
Print ISBN: 978-0-89603-386-3
Online ISBN: 978-1-59259-481-8
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