Abstract
The immobilization of proteins is an essential step in constructing affinity purification or enzyme reactor systems. For these biotechnological processes, immobilized proteins can be used in either batchwise or column operations. Commonly utilized insoluble supports for protein immobilization are beads of crosslinked gels of agarose, dextran, or synthetic polymers. When used in a batchwise mode, the beads are not easy to handle (harvesting, and so on). When used in a packed column, loosely crosslinked gels may exhibit structural instability under high pressure conditions (1), thus failing to produce high flow rates desired by biotechnological processes. Highly crosslinked gels, although structurally more stable, may exhibit lower degrees of protein immobilization within the interior of the beads, thereby reducing the reactor surface area. As an immobilization support, cellulose fibers are much less expensive than crosslinked gels and they possess a large surface area allowing for high protein loading. The fibers, however, are compressible and do not yield high flow rates when compressed into random configurations.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Janson, J. (1984) Large-scale affinity purification—state of the art and future prospects. Trends Biotechnol. 3, 23–26.
Howlett, J. R., Armstrong, D. W., and Yamazaki, H. (1991) Carbonyldiimidazole activation of a rayon/polyester cloth for covalent immobilization of proteins. Biotechnol. Techn. 5, 395–400.
Boyd, S. and Yamazaki, H. (1993) Tosyl chloride activation of a rayon/polyester cloth for protein immobilization. Biotechnol. Techn. 7, 277–282.
Yarmush, M. L. and Colton, C. K. (1985) Affinity chromatography, in Comprehensive Biotechnology (Moo-Yang, M., ed.), Pergamon, Toronto, pp. 507–521.
Nilsson, K. and Mosbach, K. (1980) p-Toluenesulfonyl chloride as an activating agent of agarose for the preparation of immobilized affinity ligands and proteins. Eur. J. Biochem. 112, 397–402.
Harlow, E. and Lane, D. (1988) Antibodies — A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 535,536.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Humana Press Inc , Totowa, NJ
About this protocol
Cite this protocol
Yamazaki, H., Boyd, C.S. (1997). Activation of Rayon/Polyester Cloth for Protein Immobilization. In: Bickerstaff, G.F. (eds) Immobilization of Enzymes and Cells. Methods in Biotechnology, vol 1. Humana Press. https://doi.org/10.1385/0-89603-386-4:77
Download citation
DOI: https://doi.org/10.1385/0-89603-386-4:77
Publisher Name: Humana Press
Print ISBN: 978-0-89603-386-3
Online ISBN: 978-1-59259-481-8
eBook Packages: Springer Protocols